摘要
以铁线莲属植物的叶片为材料,采用CTAB-SDS法、简易CTAB法、改良CTAB法等3种提取方法,比较耗时、纯度、得率、浓度和质量等指标,以期为铁线莲属植物叶片总DNA的提取筛选适宜方法。结果表明,在纯度和浓度方面CTAB-SDS法>简易CTAB法>改良CTAB法,在节省提取时间方面改良CTAB法>CTAB-SDS法>简易CTAB法。以CTAB-SDS法所提取的9种铁线莲DNA为模板,进行PCR扩增,CTAB-SDS法提取的DNA得率高、质量好,PCR扩增成功率也较高。综合比较,采用CTAB-SDS法进行铁线莲属叶片总DNA提取效果更佳。
In order to screen the ideal method to extract genomic DNA from the leaves of Clematis, 9 species of the Clematis were used as the experimental material in this study. Depending upon the index of DNA purity, yield, concentration and time consuming, three methods, CTAB-SDS, simplified CTAB, modified CTAB was compared to achieve the optimal one. The results showed that in the respect of purity, yield, concentration, the order from high to low quality was CTAB-SDS, simplified CTAB and modified CTAB, respectively; and the time consuming order from more to less time was the modified CTAB, CTAB-SDS, and simplified CTAB. The quality of genomic DNA extracted by CTAB-SDS was better than others for PCR amplification template preparing. In conclusion, the CTAB-SDS was the most suitable method for genomic DNA extraction from the leaves of Clematis.
出处
《林业科技开发》
北大核心
2014年第2期16-19,共4页
China Forestry Science and Technology
基金
江苏高校优势学科建设工程项目资助(PAPD)
