摘要
目的探讨不同浓度A型肉毒毒素（botulinum toxin typeA，BTXA）对瘢痕内成纤维细胞中仅平滑肌肌动蛋白（α smooth muscle actin，α-SMA）和肌球蛋白-Ⅱ（myosin-Ⅱ）表达水平的影响。方法从手术切除的挛缩性瘢痕组织中分离培养原代成纤维细胞，选取3—5代成纤维细胞随机分为正常组、低浓度BTXA组（1．0U／10^6 cells）和高浓度BTXA组（2．5U／10^6cells），于药物作用后1、4、7d观察成纤维细胞的生长状况，并采用Wsternblot对成纤维细胞中01．SMA和myosin-Ⅱ的表达进行测定。结果正常组成纤维细胞生长状态良好；低浓度BTXA组第4天时细胞增殖速度减慢；高浓度BTXA组第4天时细胞部分出现核固缩，第7天时细胞排列较疏松，视野呈现较多凋亡的细胞。α-SMA和myosin—Ⅱ表达在第4天时正常组显著高于BTXA作用组（P〈0．05），第7天时低浓度BTXA组与高浓度BTXA组间比较，差异有统计学意义（P〈0．05）。结论BTXA能够促进成纤维细胞凋亡，减少成纤维细胞中n—SMA和myosin—Ⅱ的表达，且在一定范围内，浓度越高抑制作用越明显。
Objective To investigate the effects of botulinum toxin type A （BTXA） on the expression of α smooth muscle actin（α-SMA）and myosin-Ⅱ of fibroblasts in scars. Methods Fibroblasts were isolated from tissue specimens of scars contracture, Cells from passages 3-5 were randomly divided into 3 groups （control group, low BTXA group （1 U/10^6Cells）, and high BTXA group （2.5 U/ 10^6Cells） ）. Growth condition of fibroblasts was observed at 1, 4, 7 day after BTXA treated. Changes of α- SMA and myosin- Ⅱ in fibroblasts were detected by Western blot. Results Fibroblasts grew well in control group, The proliferation was decreased 4 days later in BTXA groups. Lots of apoptotic ceils were seen in high BTXA group at 7th day. Proteins of α-SMA and myosin-Ⅱ in fibroblasts were statistically different between BTXA group and control groups at 4th day （P 〈 0.05）. The expression of α-SMA and myosin- Ⅱ in low BTXA group was higher than that in high BTXA group at 7th day（P 〈 0.05）. Conclusions BTXA could induce the apoptosis of fibroblasts and decrease the expression of α-SMA and myosin-Ⅱ in fibroblasts. The inhibitory effect was strengthened with BTXA concentration increase within a certain range.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2014年第2期118-121,共4页
Chinese Journal of Plastic Surgery
