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人蛔虫和猪蛔虫线粒体nad5基因的序列分析 被引量：8

Ascaris lumbricoides and Ascaris suum represent the same species based on sequence analysis of mitochondrial nad5 gene
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摘要 以采自中国不同地方的人蛔虫与猪蛔虫为研究对象，PCR扩增其线粒体烟酰胺脱氢酶亚基V基因（nad5）的部分序列]（pnad5）并进行序列测定，应用ClustalX1．81程序对序列进行比对。结果显示：所获得的pnad5序列长度一致，均为556bp；人蛔虫和猪蛔虫的pnad5序列差异仅为0．0％～2．6％，本研究结果支持人蛔虫与猪蛔虫是同一个种的结论。 Ascaris lumbricoides （A. lumbricoides） and Ascaris suum （A. suum） were collected from different areas in China to examine the sequence variation of mitochondrial NAPH dehydrogenase subuit 5 gene （nad5） by amplifying partial nad5 （pnad5） followed by sequence analysis. The results showed that the length of pnad5 sequences was 556 bp, sequence differences in the pnad5 was 0.0%-2.6% between the A. lumbricoides and A. suum samples. These results support that the A. lumbricoides and A. suum represent the same species.
作者 吴昌义 林瑞庆 朱艳平 刘国华
机构地区 重庆市璧山县养殖业技术服务中心 华南农业大学兽医学院 新乡学院生命科学与技术系 湖南农业大学动物医学院
出处 《湖南农业大学学报（自然科学版）》 CAS CSCD 北大核心 2014年第1期60-63,共4页 Journal of Hunan Agricultural University(Natural Sciences)
基金 教育部"长江学者和创新团队发展计划"项目(IRT0723)
关键词 人蛔虫 猪蛔虫 线粒体DNA 烟酰胺脱氢酶亚基V基因(nad5) 序列分析 Ascaris lumbricoides Ascaris suum mitochondrial DNA had5 sequence analysis
分类号 Q958.9 [生物学—动物学]
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参考文献23

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二级参考文献26

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湖南农业大学学报（自然科学版）
2014年 第1期

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