摘要
从山西某煤矿的酸性矿井水中分离得到嗜酸氧化硫硫杆菌Tt-1,对其形态、生理生化特性、16S rDNA基因序列、生长特性及对城市污泥的沥滤效果进行了研究。Tt-1的菌细胞呈短杆状,革兰氏染色为阴性,不产生芽孢,16S rDNA系统学分析证明其为嗜酸氧化硫硫杆菌。菌株Tt-1的最佳生长条件:温度为28℃,pH值为4.0,接种量为10%,S0浓度为10 g/L。菌株Tt-1在污泥浓度为10%、28℃的条件下沥滤15 d,对污泥中Cd、Cr、Cu、Ni、Pb和Zn的最大去除率分别为66.20%、41.35%、91.74%、65.73%、43.86%和93.72%,处理后污泥中的重金属含量可满足污泥农用标准。
A new strain of Acidithiobacillus thiooxidans Tt-1 was isolated from the acid mine drain- age of a coal mine in Shanxi Province. The morphology, biochemical and physiological characters, 16S rDNA gene sequence and growth characteristics of Tt-1 were studied. The result showed that the bacteria cell was rod-shaped and gram-negative. However, spore formation was not observed. A phylogenetic a- nalysis based on 16S rDNA gene sequence indicated that Tt-1 was Acidithiobacillus thiooxidans. Tt-1 was inoculated into the sewage sludge to bioleach heavy metals. The optimum growth conditions for Tt-1 were 28 ℃, 4.0 pH, 10% inoculation rate and 10 g/L S0. After bioleaching the sewage sludge using strain Tt-1 at 28℃ and 10% sewage sludge concentration for 15 d, the removal rates of Cd, Cr, Cu, Ni, Pb and Zn were 66.20%, 51.35%, 91.74%, 65.73%, 43.86% and 93.72%, respectively. The total contents of six heavy metals in the treated sludge could meet Chinese quality standards for agriculturalland utilization of sewage sludge.
出处
《中国给水排水》
CAS
CSCD
北大核心
2014年第7期21-26,共6页
China Water & Wastewater
基金
国家自然科学基金资助项目(41161075)
广西"八桂学者"建设工程专项经费资助项目
"广西危险废物处置产业化人才小高地"资助项目
关键词
嗜酸氧化硫硫杆菌
生长特性
污泥
生物沥滤
Acidithiobacillus thiooxidans
growth characteristics
sewage sludge
bioleach- ing
