摘要
目的:研究RAB5A基因转染后对肺腺癌侵袭能力的影响。方法:采用重基底膜侵袭实验,观察转染前后的细胞系对基底膜侵袭能力的改变。结果：转染PcDNA3.1-RAB5A正义表达载体的AGZY83-a细胞系基底膜侵袭能力增强，转染PcDNA3-AntiRAB5A反义RNA组质粒的Anip973细胞系侵袭能力明显减弱。结论：RAB5A在人非小细胞肺癌的侵袭及转移特性的形成中具有重要的作用，RAB5A的反义分子能够有效地阻断该基因表达的翻译过程，在细胞内发挥预期作用。
Object to investigate the effect of RAB5A gene on forming of tumor metastatic nature.Method: used reconstituted basement membrane invasion technique.Results: the results suggest that invasiveness of AGZY83-a cells of transfected PcDNA3.1-RAB5A was higher than parent line,and Anip973 cells of transfected PcDNA3-AntiRAB5A was lower than Anip973 cells Conclusion: results of our research in vitro indicate that RAB5A gene over-expression plays important role in tumor invasion phenotype formation metastatic nature of human lung adenocarcinoma cells.Antisense RNA can interrupt the translation of RAB5A,and exert the expect effects.
出处
《牡丹江医学院学报》
2000年第4期4-6,共3页
Journal of Mudanjiang Medical University
