摘要
目的：观察分析生长抑素类似物（善宁）对急性胰腺炎大鼠胰腺组织表皮生长因子（ＥＧＦ）ｍＲＮＡ表达、ＤＮＡ合成、总蛋白含量的影响及相互关系，进一步探讨善宁治疗急性胰腺炎的作用机制。方法：以腹腔注射雨蛙肽诱导大鼠急性胰腺炎模型，并分别于诱导后６ｈ、２４ｈ、４８ｈ、７２ｈ和９６ｈ时处死大鼠。用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）技术检测胰腺组织ＥＧＦｍＲＮＡ表达，用３Ｈ－胸腺嘧啶核苷体外掺入法测定胰腺组织ＤＮＡ合成状态，用Ｌｏｗｒｙ’ｓ法测定胰腺组织总蛋白含量。结果：善宁治疗组大鼠血清淀粉酶水平较非治疗组显著下降。正常胰腺组织和胰腺炎诱导后６ｈ的胰腺组织未见ＥＧＦｍＲＮＡ表达，但诱导后２４ｈ至９６ｈ均可检测到ＥＧＦｍＲＮＡ表达；善宁治疗后４８ｈ，ＥＧＦｍＲＮＡ表达较非治疗组明显增强。胰腺炎诱导后７２ｈ，非治疗组大鼠胰腺组织ＤＮＡ合成明显下降；善宁治疗后９６ｈ，ＤＮＡ合成较非治疗组明显增加。胰腺炎诱导后４８ｈ，非治疗组大鼠胰腺组织总蛋白含量明显下降；善宁治疗后４８ｈ，总蛋白含量较非治疗组明显增加，至９６ｈ时达最高值。结论：善宁治疗急性胰腺炎的可能机制是诱导胰腺组织ＥＧＦ表达增强，刺激胰腺细胞增生，增加胰腺组织ＤＮＡ合成?
Background/Aims:To investigate the effects and mechanisms of somatostatin analogues (San- dostatin) on acute pancreatitis of rats by analyzing the changes of pancreatic tissue epidermal growth factor (EGF) gene expression, DNA synthesis, total protein content, and the relationship among them. Methods: Acute pancreatitis was induced by intra-peritoneal infusion of caerulein in rats, Sandostatin was administered intra-peritoneally at the time of induction and 24, 48 and 72 hours afterwards. Rats were sacrificed at 6, 24, 48, 72 or 96 hours after the induction. The mRNA expression for EGF was evalu- ated by reverse transcription polymerase chain reaction (RT-PCR), pancreatic tissue DNA synthesis was measured by3 H-thymidine method in vitro and total protein content was detected by Lowry's method. Results: The serum amylase level decreased significantly in the Sandostatin-treated group in comparison with the non-treated group. Expression of EGF mRNA was undetectable in the intact pan- creas and the non-treated group at 6 hours but could be observed from 24 hours up to 96 hours after the induction of pancreatitis. The expression of EGF mRNA increased significantly in the Sandostatin- treated group as compared with the non-treated group at 48 hours. Pancreatic tissue DNA synthesis showed a significant decrease in the non-treated group during the first 72 hours following the induc- tion, but a marked increase was observed at 96 hours after the treatment with Sandostatin. During 48 hours following the induction, pancreatic tissue total protein content declined significantly in the non- treated group, and there was a remarkable increase in the Sandostatin-treated group at 48 hours, reach- ing a maximum at 96 hours. Conclusions: Mechanisms of Sandostatin in treating acute pancreatitis might be attributed to the enhancement of EGF gene expression which subsequently stimulates pan- creatic cell proliferation, increases DNA synthesis and protein content, and accelerates the pancreatic repair and remodeling.
出处
《胃肠病学》
2000年第4期216-219,共4页
Chinese Journal of Gastroenterology
关键词
胰腺炎
生长抑素
表皮生长因子-尿抑胃素
基因表
Pancreatitis
Somatostatin
Epidermal Growth Factor-Urogastrone
Gene Expression
