摘要
目的 探讨缺氧、缺血对新生鼠大脑组织天冬氨酸特异酶切的半胱氨酸蛋白酶 3(简称caspase 3)活性的影响及意义。方法 选择 7d龄Sprague Dawley大鼠 33只 ,结扎左侧颈总动脉后 ,吸入 8%氧气 2h ,制成缺氧、缺血性脑损伤 (HIBD)动物模型 ,HIBD后 0 5、12、2 4、48h取两侧大脑组织制成组织匀浆 ,用显色底物天冬氨酸 谷氨酸 缬氨酸 天冬氨酸 对硝基苯胺复合物测定caspase 3活性。结果 缺氧、缺血侧大脑组织caspase 3活性逐渐增高 ,2 4h后吸光度值达 1 0 0 47± 0 4390 ,48h后降至 0 45 6 9± 0 2 2 85 ,各时点间差异有极显著性 (P <0 0 0 1)。对侧大脑组织各时间点caspase 3活性未见明显变化 (P >0 0 5 )。结论 新生鼠大脑缺氧、缺血后caspase 3明显激活 ,支持细胞凋亡参与HIBD ,应用caspases抑制剂或其他抗凋亡药物 。
Objective To study the effect of hypoxia ischemia on cysteinyl aspartate specific proteinases (caspase 3) activity in cerebral tissue of neonatal rat and probe into its significance Methods To induce hypoxic ischemic brain damage (HIBD), the left carotid artery of rats at day 7 was ligated and animals were exposed to 8% oxygen for 2 hours 0 5,12,24,and 48 hours after HIBD, both ipsilateral and contralateral cerebral tissue were ditected and homogenized caspase 3 activity was measured by cleavage of the colorimetric substrate DEVD pNA Results Caspase 3 activity in ipsilateral cerebral tissue increased gradually after HIBD and peaked at 24 hours, and then decreased significantly at 48 hours( P <0 001) There were no significant changes in caspase 3 activity in the contralateral tissue at all time points ( P >0 05) Conclusions Significant activation of caspase 3 after cerebral hypoxia ischemia strongly suggests that apoptosis is involved in HIBD Application of caspase inhibitors or other anti apoptotic agents may become a new therapeutics of HIBD
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2001年第2期79-81,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
半胱氨酸蛋白酶类
脑缺氧
脑缺血
脱噬作用
Cysteine proteinases
Cerebral anoxia
Cerebral ischemia
Apoptosis
