摘要
目的研究溴氰菊酯(DM)对大鼠脑组织线粒体膜通透性及细胞色素C表达的影响。方法Wistar大鼠随机分为实验组(4个不同染毒时间)和对照组,每组5只。实验组按12．5 mg/kg DM一次腹腔注射,5、24、48、72 h后处死；对照组一次腹腔注射5 mg/kg的色拉油,分别提取大鼠大脑皮层和海马的线粒体,测定线粒体膜通透性、细胞色素C氧化酶,并测定大鼠大脑皮层、海马CA1、CA2、CA3、CA4区细胞色素C的表达。结果实验组与对照组相比,5、24、48、72 h组的线粒体膜通透性增大；皮层和海马CA1区、CA2区5、24、48 h组(0．57±．04、0．67±0．09、0．58±0．04)、CA4区24 h组(0．81±0．18)细胞色素C表达增强,吸光度值的差异有统计学意义(P＜0．05或P＜0．01)；CA2区72 h组、CA3区及CA4区5、48、72 h组细胞色素C表达,吸光度值的差异则无统计学意义(P＞0．05)；细胞色素C氧化酶的活力则受到抑制,差异有统计学意义(P＜0．01)。结论DM能明显增加大鼠脑组织线粒体膜通透性,促使细胞色素C表达增强。
Objective To study the effects of deltamethrin（DM） on the permeability of mitocbendrial membrane and the expression of cytochrome C in brain tissue of rats. Methods Wistar rots were randomizedly ditided into five groups（including four treated groups and one control group）. In the treated groups, DM of 12.5 mg/kg was administered intraperitoneally once in rats and the rots were sacrificed 5,24,48 and 72 hours laterwhile in the control group, the salad oil of 5 mg/kg was administered intraperitoneally once. The mitochondria in brain tissue of rats were extracted to measure the membrane permeability and the activity of cytochrome C oxidase as well as the expression of cytochrome C in cortex and hippocampus. Results After the treatment the permeability of mitochondrial membrane was significantly increased in the treated groups compared with the control group. The expression of cytochrome C was increased in cortex and hippocampus CA1 and CA2 5 h,24 h and 48 h groups and CA4 24 h group（0.57 ± 0.04,0.67 ± 0.09,0.58 ± 0.04） and（0.81 ±0.18）（P〈 0.05 or P 〈 0.01） while there was no significant difference in the expression of cytochrome C in cortex and hippocampus CA2 72h group and CA3 and CA4 5 h, 48 h and 72 h groups between the treated groups and the control group（ P 〉 0.05）. The activity of cytochrome C oxidase was inhibited（ P 〈 0.01）. Conclusion Deltamethrin can significantly increase the permeability of mitochondrial membrane and the expression of cytochrome C in brain tissue of rats.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2006年第6期326-329,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金(30371225)
