摘要
目的 探讨高热惊厥是否能引起发育期大鼠脑损伤。方法 采用热水浴诱导大鼠高热惊厥模型。发育期大鼠随机分为两组 :正常对照组 (n =10 )和高热处理组 (n =35 )。根据大鼠是否有惊厥 ,将高热处理组 (5只在实验过程中死亡 )又分为高热未惊厥组 (n =10 )和高热惊厥组 (n =2 0 )。电镜观察各组大鼠海马神经元超微结构改变 ,体视学方法计数各组大鼠海马CA1区神经元数密度。结果 高热惊厥大鼠海马CA1区和门区神经元线粒体体积减小 ,基质浓缩 ,嵴模糊不清或消失 ,部分线粒体出现空泡 ;高尔基复合体轻 中度扩张。高热惊厥组大鼠海马CA1区神经元数密度 [(1 2 2±0 13)× 10 -4 μm-3]显著减小 ,与正常对照组 [(1 79± 0 11)× 10 -4 μm-3]及高热未惊厥组 [(1 84±0 13)× 10 -4 μm-3]比较 ,差异均有显著性 (P <0 0 1) ;高热未惊厥组与正常对照组比较 ,海马CA1区神经元数密度差异无显著性 (P >0 0 5 )。
Objective Febrile convulsion (FC) is one of the most common seizure disorders in children. About 3% of the patients with FC develop epilepsy later. Clinical studies have demonstrated a close relationship between FC and temporal epilepsy. Previous studies on FC were mainly focused on the clinical and electroencephalographic observations. Few neuropathological studies was reported. The main purpose of the present study was to find out whether FC causes the brain damage in developing rats. Methods Warm water induced rat FC model was developed in this study. Forty five rats were randomly divided into two groups: normal control group ( n =10) and hyperthermia treated group ( n =35). The latter was further divided into FC and hyperthermic (non convulsive) groups according to the reaction to hyperthermia treatment. Four rats from each group were selected for the further studies. The ultrastructural changes of the hippocampal neuron were observed under the electron microscope, and the numerical density of the hippocampal CA 1 neurons was counted by means of the dissector system. Results In the hyperthermia treatment group, twenty rats developed FC (FC group), ten did not develop FC. Five died of the first hyperthermia treatment. Each rat in the FC group was induced 10 convulsions, with an interval of 2 days. No special ultrastructual findings were observed from the hippocampal CA 1 and hile neurons in the normal and the hyperthermic groups. In FC group, however, the mitochondrion volume decreased, the matrix condensed, the ridge obscured or disappeared, and vacuoles formed in some mitochondria. Mild to moderate dilation of Golgi′s complex was also observed. Neuronal numerical density (Nv) in hippocampal CA 1 in FC group [(1. 22±0. 13)×10 -4 μm -3 ] significantly decreased ( P <0. 01)compared with that in normal [(1 79±0 11)×10 -4 μm -3 ] and hyperthermic [(1. 84±0. 13)×10 -4 μm -3 ] groups. No significant difference of Nv was observed between normal group and hyperthermic group ( P >0. 05). Conclusion The warm water induced FC model appeared to be simple and feasible for long term study. Recurrent FC may cause damage and loss of hippocampal neuron in the developing rats.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2001年第2期105-108,共4页
Chinese Journal of Pediatrics
