摘要
本试验旨在研究白花蛇舌草超微粉对小鼠免疫功能的影响。试验将60只昆明小鼠随机等分为4组，高、中、低剂量组（1～3组）分别以小鼠每千克体重灌胃不同剂量白花蛇舌草超微粉悬液，剂量依次为1．75、1．25、0．75g／kg，连续给药7d；第4组为空白对照组，灌胃等量的生理盐水。最后一次给药后间隔12h，各组小鼠用鸡新城疫弱毒疫苗接种，随后在给药结束后的第7、14和21天采集小鼠外周血用于分离淋巴细胞和血清，用CCK-8法测定外周血淋巴细胞增殖能力，微量血凝抑制试验评价血清中新城疫抗体水平；给药结束后21d测定小鼠腹腔巨噬细胞吞噬能力，并测定脾脏指数。结果显示，高剂量白花蛇舌草超微粉可极显著提高小鼠外周血淋巴细胞增殖能力、免疫抗体水平（P〈0．01），同时还能极显著提高腹腔巨噬细胞的吞噬能力和脾脏指数（P〈0．01）。试验结果表明白花蛇舌草超微粉能极显著提高机体的特异性免疫和非特异性免疫功能，为以后开发白花蛇舌草超微粉成为新型天然植物药、饲料添加剂提供了依据。
The assay was aimed to study the effect of Hedyotis diffusa Willd superfines powder on immune function. Three groups of mice were gavaged with the drugs at doses of 1.75,1.25 and 0.75 g/kg once a day for 7 consecutive days, re- pectively. The mice of control group were administered with the same volume of saline. At the last time, mice were injected into the muscle with Newcastle disease vaccine,and get peripheral blood on days 7, 14 and 21 after the medicine administration. The enhancement of innate immune responses were evaluated by using CCK 8 method, hemagglutination inhibition test, macrophage phagocytic function test and organ coefficient method. The levels of lymphocytes proliferative capacity, serum antibody, perito heal macrophage phagocytosis and the indexes of immune organs in mice were extremely significantly enhanced （P〈0.01）. Hedyotis diffusa Willd superfines powder could extremely significantly regulate the immunity function of mice （P〈0.01）.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第3期166-169,共4页
China Animal Husbandry & Veterinary Medicine
基金
"十二五"国家科技支撑计划(2011BAD34B02-4)
关键词
白花蛇舌草
超微粉
小鼠
免疫功能
Hedyotis diffusa Willd
superfines powder
mouse
immune function
