摘要
用聚苯乙烯石蜡体系制备涂膜聚苯胺尿酸酶电极的方法简单 ,在 0.35 V（ vs SCE)时的响应时间仅为 30 s左右 .涂膜聚苯胺尿酸酶电极酶催化反应的最适 pH和电势依赖性与聚苯胺尿酸酶电极酶催化反应的最适 pH和电势依赖性相似 ,但涂膜聚苯胺尿酸酶电极酶催化反应的活化能（ 19.9 kJ· mol－ 1）较无膜聚苯胺尿酸酶电极酶催化反应的活化能（ 29.9 kJ· mol－ 1）低 ,而前者较后者的稳定性好（前者超过 180天） .
A simple doping-coating technique is described for immobilizing uricase using polyaniline and polystyrene and paraffin, The polyaniline uricase electrode with polystyrene coating has a fast response time (about 30 s at 0.35 V vs. SCE). The optimum pH and the dependence on potential are similar to those of polyaniline uricase electrode, the activation energy and stability of the polyaniline uricase electrode with polystyrene coating are 19.9 kJ . mol(-1) and over 180 days, respectively.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
2001年第1期32-36,共5页
Acta Physico-Chimica Sinica
基金
国家自然科学基金! (20074027)
