摘要
用聚苯胺尿酸酶电极研究了茶碱对固定酶的影响．结果表明，茶碱对固定尿酸酶有明显的抑制作用，但这种抑制作用是可逆的．在茶碱的存在下，pH对酶电极的响应电流影响与无茶碱存在时不同；在0．2－0．5V之间，酶电极的响应电流随电位增加而迅速升高，当电位进一步升高时，其变化速率减慢．茶碱使尿酸酶催化反应的活化能从无抑制剂存在时的29．9kJ·mol-1提高到47．8kJ·mol-1，即抑制剂改变了尿酸酶催化反应的途径．
The effect of theophylline on the immobilized enzyme has been investigated using polyaniline uricase electrode. The activity of the immobilized uricase is markedly inhibited by theophylline. However, this is a reversible inhibition. In the presence of theophylline, the effect of pH on the response current of the enzyme electrode is different from that without theo-phylline, the response current of the enzyme electrode rapidly increase with increasing potential from 0.20-0.50V, and then increases slowly with further increasing potential. The activation en-ergy of the enzyme-catalyzed reaction is 47.8kJ.mol-1 in the presence of theophylline, which is much larger than that without theophylline (29.9kJ.mol-1). This indicates that the path of the enzyme-catalyzed reaction was changed by theophylline.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
1997年第3期236-241,共6页
Acta Physico-Chimica Sinica
基金
中国科学院化学研究所有机固体开放实验室资助
关键词
催化活性
聚苯胺
尿酸酶
茶碱
抑制作用
Polyaniline, Uricase, Theophylline, Inhibition, Activation energy
