摘要
为研究制备载羟基喜树碱的叶酸-壳聚糖纳米冻干粉的制备方法,以羟基喜树碱、叶酸、壳聚糖、三聚磷酸钠为原料,以葡萄糖和甘露醇为保护剂,应用真空冷冻干燥技术,制备了载羟基喜树碱的叶酸-壳聚糖纳米粒冻干粉.以冻干粉外观及在水中再分散性为指标,分别考察了冻干保护剂的种类、体积分数、用量及加入方式对冻干的影响.并利用扫描电镜及透射电镜对冻干粉的微观形态进行观察.结果表明,甘露醇作为冻干保护剂优于葡萄糖.当甘露醇体积分数为8%,与纳米粒水分散体系体积比为1∶1时,所制备的冻干粉呈颜色均一、外表光滑、致密松脆的完整的饼状,且在水中再分散迅速,无任何悬浮物,成均一乳状胶体.在扫描电镜下观察冻干粉呈花瓣形,再分散后于透射电镜下观察,纳米粒子呈规则球形,大小均匀,平均粒径约200 nm左右.该工艺所制备得冻干粉针剂有望成为羟基喜树碱的新剂型.
To research the preparation method of lyophilized powder of hydroxycamptothecin using hydroxycamptothecin , folic acid, chitosan and sodium tripolyphosphate as raw materi-als, glucose and mannitol as cryoprotectants , the nanoparticles lyophilized powder was pre-pared with the method of vacuum freeze drying technology .With the appearance and redis-persibility in water of lyophilized powder as examining index , the factors were respectively investigated , including the types , concentration , amount and adding method of the cryopro-tectants .The morphology of the lyophilized powder was observed by scanning electron micro-scope （SEM） and transmitting electron microscope （ TEM） respectively.Results indicated that as cryoprotectant mannitol was better than glucose .When mannitol concentration was 8%, and the volume ratio of nanoparticles water dispersion system to mannitol solution was 1∶1, the lyophilized powder was the whole pie with uniformly yellow , smooth, compact crunchy .Furthermore , it dispersed quickly into a homogeneous emulsion colloid without any suspended substance in distilled water .The morphology of lyophilized powder was regularly＆amp;nbsp;spherical and uniform with the average grain diameter of 200 nm under TEM , and appeared petaling under the SEM .The lyophilized powder injection prepared by the process is expec-ted to become a new pharmaceutical dosage form of hydroxycamptothecin .
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2014年第1期13-17,共5页
Journal of Harbin University of Commerce：Natural Sciences Edition
基金
黑龙江省教育厅科研项目(12531156)
