摘要
经过紫外扫描确定吡虫啉和啶虫脒的最大紫外吸收波长分别为270和246 nm，通过改变其比例优化出峰时间和分离度，确定V（乙腈）∶V（水）＝3∶7为流动相，建立了两种农药的标准曲线，结果表明在0.05～5μg· mL^-1范围内线性关系均良好。进行样品加标回收实验，用乙腈提取，C18固相萃取柱净化，上述条件进行下分析，0.1，0.5，1.5 mg·kg^-1三个加标浓度回收率均在75％～110％，变异系数＜5％，准确性和稳定性都较好，方法最低定量限0.01μg·g^-1，完全可以用于实际样品的检测。
The maximum absorption wavelength of imidacloprid and nitenpyram detected by UV spectrophotometer were 270 nm and 246 nm.To get optimum retention time and separation , the mobile phase should be acetonitrile and water （ V/V, 3∶7 ） .Standard curves of two pesticides were developed , which fit a linear regression ranging from 0.05 to 5 μg· mL^-1 .After extracted by homogenate with acetonitrile and purified by C 18 SPE column, the recovery rates of the two pesticides were 75%~110% at the levels of 0.1mg·kg^ -1 , 0.5mg· kg^-1 and 1.5mg＆#183; kg -1 with a RSD less than 5%, which showed a good accuracy and stability .This method with a limit of quantification （ LOQ） of 0.01μg· g^-1 could be used in the practical detections .
出处
《浙江农业学报》
CSCD
北大核心
2014年第1期131-134,共4页
Acta Agriculturae Zhejiangensis
基金
中央级公益性科研院所基本科研业务费(2011Hzs1J006
2010hzsZDZX001)
