摘要
为探索适合桑黄总RNA的提取方法,以桑黄菌丝体为试验材料,比较了用Trizol法、CTAB法和RNA prep pure Plant Kit试剂盒法提取后总RNA的质量。结果表明,3种方法均能从桑黄菌丝体中提取分离到总RNA,其中Trizol法能提取到纯度较高、完整性较好的总RNA,28S及18S带型清晰无弥散,A260/A280值为1.96,RNA产率为604.64μg·g-1,提取的总RNA适用于基因克隆与基因表达分析等分子生物学试验研究。
In order to obtain an ideal total RNA extraction method of Phellinus baumii, total RNA of Phellinus baumii was extracted with 3 methods including Trizol method , CTAB method and RNA prep pure Plant Kit method .The re-sults showed that all these methods can extract total RNA from Phellinus baumii, however , the Trizol method was the best and had high integrity and purity .The band of 28S and 18S was clear.The value of A260/A280 was 1.96 and the RNA yield was 604.64 μg· g^-1 .Thus, the RNA extracted by Trizol method could be used for RT-PCR, gene cloning and gene expression and so on .
出处
《浙江农业学报》
CSCD
北大核心
2014年第1期95-99,共5页
Acta Agriculturae Zhejiangensis
基金
黑龙江省教育厅科学技术研究项目(1551538)
关键词
桑黄
RNA提取
TRIZOL法
RT-PCR
Phellinus baumii Pilat
RNA extraction
Trizol method
RT-PCR
