摘要
目的:探索不同植物激素配比对瓦松愈伤组织诱导、增殖及分化的影响。方法:采用组培技术,以种子萌发的无菌苗叶片为外植体,采用正交试验研究IAA、NAA、6-BA、KT对愈伤组织诱导及增殖的影响,探索适宜愈伤组织分化的培养基。结果:愈伤组织诱导的最佳培养基为MS+IAA 1.0 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L,增殖的最佳培养基为MS+IAA 0.5 mg/L+6-BA 0.5 mg/L+KT 1.0 mg/L,MS+IAA 0.1 mg/L+KT 2.0 mg/L为愈伤组织分化不定芽的最佳培养基,1/2 MS+IAA 0.2 mg/L培养基有利于不定根分化。结论:利用组织培养技术成功建立了瓦松的植株再生体系。
Objective：To explore the effects of different hormonal combinations on induction,proliferation and differentiation of Orostachyis fimbriatae callus culture.Methods：Aseptic seedling leaves were used as explants,the different concentrations of IAA,NAA,6-BA and KT on induction proliferation of callus were optimized by orthogonal test to explore the optimum medium for differentiation of callus by tissue culture techniques.Results：The best medium for induction was MS＋IAA 1.0 mg/L＋NAA 0.5 mg/L＋KT 1.0 mg/L,and the best hormonal combination for proliferation was MS＋IAA 0.5mg/L＋6-BA 0.5 mg/L＋KT 1.0 mg/L.The best medium for differentiation was MS＋IAA 0.1 mg/L＋KT 2.0 mg/L,and 1 /2MS＋IAA 0.2 mg/L was the optimum medium for rooting culture.Conclusion：The system of regeneration of Orostachyis fimbriatae is establishd by tissue culture techniques in this study.
出处
《中药材》
CAS
CSCD
北大核心
2014年第1期1-4,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(31100246)
四川省科技厅项目(2013JY0078)
绵阳师院校级项目(2012A08)
生态安全与保护四川省重点实验室开放基金项目(ZDS1004)
关键词
瓦松
叶片
愈伤组织
分化
植株再生
Orostachyis fimbriatae Berger
Leaf
Callus
Differentiation
Regeneration
