摘要
为探究银杏营养贮藏蛋白质的结构与功能的关系,采用DEAE-Sepharose FF离子交换色谱和Sephadex G-75葡聚糖凝胶色谱对银杏营养贮藏蛋白进行分离纯化,得到一条36 kDa的蛋白。质谱分析结果表明:该蛋白中的2个肽段序列与大豆贮藏蛋白质(29 kDa)和薯蓣块茎贮藏蛋白质(30 kDa)的部分氨基酸序列吻合。红外光谱结果表明:β-折叠构象是银杏营养贮藏蛋白质的主要二级结构。
To reveal the relationship between the structure and function of vegetative storage proteins in Ginkgo biloba, the vegetative storage proteins were separated and purified by DEAE-Sepharose FF ion-exchange chromatography and Sephadex G-75 gel chromatography. A target protein was obtained and its molecule weight was 36 kDa. The mass- spectrometry showed that there were two peptide sequences which were consistent to some sequences of the Soybean storage protein （29 kDa） and the Dioscorea storage protein （30 kDa）. The infrared spectrometry indicated that the secondary structure of the protein was mainly in β-sheet conformations.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2014年第2期37-41,共5页
Scientia Silvae Sinicae
基金
江苏省科技支撑(农业)项目(BE2011381)
江苏省普通高校研究生科研创新计划项目(CXZZ12-0544)
江苏高校优势学科建设工程资助项目(PAPD)
关键词
银杏
营养贮藏蛋白质
分离纯化
结构鉴定
Ginkgo biloba
vegetative storage protein
isolation and purification
structure identification
