摘要
目的 观察吡咯烷二硫代氨基甲酸（PDTC）对匹鲁卡品诱导癫痫大鼠海马CA1、CA3和DG各区神经元及小胶质细胞的影响.方法 30只大鼠随机分为正常对照组（n=6）、癫痫组（n=12）及PDTC干预组（n=12）.采用一次性腹腔注射匹鲁卡品（320 mg/kg）诱导大鼠癫痫发作；PDTC干预组分别于注射匹鲁卡品前24 h、20 rmin腹腔注射PDTC（100 mg/kg）；正常对照组注射等量生理盐水.监测大鼠行为学改变；采用Fluoro-Jade C（FJC）染色法检测海马DG、CA1、CA3区退行性改变神经元；免疫组织化学方法检测各组大鼠海马各区小胶质细胞表达情况.结果 癫痫组及PDTC干预组各有10只大鼠制模成功.正常对照组大鼠无癫痫发作；癫痫组大鼠平均跌倒次数[（32.30 ±4.37）次]显著多于PDTC干预组[（17.50±2.37）次]（P＜0.05）.正常对照组大鼠海马各区未见FJC阳性细胞及少量Iba-1标记的小胶质细胞.与癫痫组比较,正常对照组DG、CA1、CA3锥体层Iba-1标记的小胶质细胞数显著减少（P＜0.01）；PDTC干预组CA1、CA3区FJC阳性细胞数及锥体层Iba-1标记的小胶质细胞数明显减少（P ＜0.05～0.01）.结论 PDTC可能通过抑制小胶质细胞活性,影响小胶质细胞介导的神经炎症反应,从而对癫痫持续状态所致脑损伤起到保护作用,改善癫痫发作的严重程度.
Objective To investigated the effect of pyrrolidine dithiocarbamate （PDTC） on neurons and microglia cells in CA1,CA3,DG of hippocampus of epileptic rats induced by pilocarpine.Methods Thirty rats were randomly divided into normal control group （n =6）,epilepsy group （n =12） and PDTC group （n =12）.The epileptic rat model was induced by injected intraperitoneally with pilocarpine 320 mg/kg.PDTC group was intraperitoneally with PDTC 100 mg/kg at 24 h and 20 min before creation of model.Normal control group accepted same volume of saline.The behavioral changes of rat were observed.The degenerating neurons in hippocampus CA1,CA3,DG were detected by Fluoro-Jade C （FJC） staining,and the expression of microglia was detected by immunohistochemical method in each group.Results The patterns successively was in 10 rats in epilepsy group and PDTC group respectively.There was no seizure in normal control group.The falling numbers in epilepsy group （32.30 ± 4.37） was significantly increased than PDTC group （17.50 ± 2.37） （P 〈 0.05）.There were no FJC positive cells and few microglia which labeled by Ibal in hippocampus in normal control group.Compared with epilepsy group,the numbers of microglia cells in CA1,CA3 and DG area of hippocampus were significantly reduced than that in normal control group （P 〈0.01） ; and the numbers of FJC positive cells in CA1,CA3 area and microglia cells in pyramidal neuron cell layer were significantly reduced （P 〈 0.05-0.01）.Conclusion PDTC may inhibit the activity of glia cell to influence glial-mediated inflammatory pathways,protect brain damage by epilepsy,and lighter the expert of epilepsy.
出处
《临床神经病学杂志》
CAS
北大核心
2014年第1期33-36,共4页
Journal of Clinical Neurology
