摘要
通过基因重组、定点突变、基因整合和诱变等手段构建了一个抗氧化型枯草杆菌碱性蛋白酶高产工程菌株B135,试管及三角瓶发酵结果显示 ,酶产量是 10 0 4 8U /ml,是2 70 9碱性蛋白酶产量的 55% ,抗氧化型碱性蛋白酶浓度是 5.9mg/ml。该酶与 1mol/L过氧化氢作用 15min后 ,酶活保存 90 %以上 ,而 2 70 9则保存 12 % ,说明B135碱性蛋白酶是抗氧化的。
An Engineering strain B315 with high production of oxidatively resistant subtilisin was constructed by gene recombination, site directed mutagenesis, gene integration and mutagenesis. The result of fermentation in triangle flask shows that the production of enzyme is 10048 u/ml, which is 55% of 2709. And the Consistency if the oxidatively resistant enzyme is 5.9 mg/ml. After reacting with H 2O 2 for 15min, the enzyme activity was remained beyond 96%of its original. But in the same conditions, the enzyme activity of 2709 subtilisin was only 12% of its original value.These indicated that the subtilisin of B135 was oxidatively resistant.
出处
《高技术通讯》
EI
CAS
CSCD
2000年第10期13-18,共6页
Chinese High Technology Letters
基金
863计划资助项目
