摘要
目的筛选同时富集锌、铜、硒、锗四种元素能力较强的酵母菌株,并确定最佳培养条件。方法通过逐步提高培养液中添加微量元素浓度的方法驯化酵母菌;运用正交试验筛选富集能力较强的菌株和其最佳培养条件。结果筛选出耐受金属离子能力较强的酿酒酵母菌株,其最佳培养条件为加锌量600μg/ml、加铜量67μg/ml、加硒量25μg/ml、加锗量60μg/ml,培养时间72 h,温度30℃,接种量15%。此条件下培养的酿酒酵母菌含锌元素浓度257.03μg/g、铜元素浓度597.19μg/g、硒元素浓度62.62μg/g、锗元素浓度1.85μg/g。结论酿酒酵母菌株能同时富集锌、铜、硒、锗四种元素,在最佳培养条件下能获得含有机微量元素较高的酵母菌。
Objective To screen the yeast strain which contained abundant zinc, copper, selenium and germanium simultane- ously, and establish the optimum cuhure conditions. Methods The yeast strain was domesticated by increasing concentrations of microelements in culture medium. Orthogonal test was used to screen the yeast strain with stronger enrichment capability and the optimum culture conditions. Results Saccharomyces cerevisiae strains were screened out, which had high tolerance capacity to metal ions. The optimum culture conditions for the amount of zinc, copper, selenium and germanium were 600 μg/ml, 67 μg/ml, 25 μg/ml and 60 μg/ml, respectively. The culture time was 72 hours, temperature was 30 ℃, and inoculation vol- ume was 15%. Under the above optimum conditions, Saccharomyces cerevisiae contained 257.03 μg/g zinc, 597.19 μg/g cop- per, 62.62μg/g selenium and 1.85 μg/g germanium. Conclusion Saccharomyces cerevisiae is able to enrich zinc, copper, selenium and germanium element together, the yeast containing higher concentrations of organic trace elements can be obtained under the optimum culture conditions.
出处
《中国卫生检验杂志》
CAS
北大核心
2014年第4期512-514,518,共4页
Chinese Journal of Health Laboratory Technology
基金
黑龙江省科技攻关项目"生物活性酵母的研制"(GC10C303-5)
关键词
微量元素
酵母
正交试验
Trace elements
Yeast
Orthogonal test
