摘要
目的:探讨宫颈腺癌组织中HPV16/18-E7、Cyclin D1/pRb及ER的表达及临床意义。方法:收集2004-01-01-2012-01-01胜利油田中心医院病理科宫颈腺癌患者40例。应用免疫组化法检测40例宫颈腺癌组织中HPV16/18-E7、Cyclin D1及ER的表达,蛋白质印迹法检测HPV16/18-E7和Cyclin D1均阳性表达的宫颈腺癌组织中Cyclin D1及pRb的表达量。结果:免疫组化结果显示,宫颈腺癌组织中Cyclin D1阳性表达率为55.0%,HPV16/18-E7为85.0%,ER为72.5%。HPV16/18-E7蛋白及ER的表达与宫颈腺癌患者的年龄、肿瘤大小、临床分期、组织学分级及淋巴结转移等临床病理特征无关,P>0.05。Cyclin D1的高表达与宫颈腺癌的临床分期有关,χ2=6.234,P=0.013;与淋巴结是否转移有关,χ2=9.038,P=0.003;与患者年龄、肿瘤大小和组织学分级无关,P>0.05。蛋白质印迹法检测显示,HPV16/18-E7和Cyclin D1均阳性表达的21例宫颈腺癌组织中Cyclin D1出现不同程度的高表达,对照组7例组织中Cyclin D1蛋白低表达或无表达,差异有统计学意义,PCyclin D1=0.037;21例宫颈腺癌组织中pRb蛋白多为低表达或不表达,对照组pRb蛋白的表达无明显变化,PpRb=0.002。宫颈腺癌组织中Cyclin D1的表达与HPV16/18-E7蛋白呈正相关,χ2=17.56,r=0.902 4,P=0.042;ER与HPV16/18-E7蛋白表达呈正相关,χ2=5.431,r=0.345 8,P=0.02;Cyclin D1的表达与ER受体呈正相关,χ2=4.713,r=0.317 1,P=0.03。结论:Cyclin D1/pRb信号通路的活化可能是HPV16/18感染宫颈腺癌的发生机制,HPV16/18阳性宫颈腺癌组织中ER高表达,提示雌激素在高危型HPV16/18致癌过程中可能发挥作用。
OBJECTIVE： To study the expression of HPV16/18-E7, Cyclin D1, pRb, ER and its clinical significance in uterine cervical adenocarcinoma. METHODS： Fourty cases of cervical adenocarcinoma in Pathology Department of Shengli Oilfield Central Hospital from January 2004 to January 2012. The expression of Cyclin D1, HPV16/18-E7 and ER was de tected in 40 cases of cervical adenoearcinoma by immunohistoehemistry. The expression of Cyclin D1 and pRb were detec ted by Western-Blot in cases of cervical adenocarcinoma in which the expression of HPV16/18 E7 and Cyclin D1 were positive. RESULTS： The result of immunohistochemical method showed that the positive expression rates for Cyclin D1 was 55.0% ,the positive expression rates for HPV16/18-E7 was 85.0%, and the positive expression rates for ER was 72.5 %. The expression of HPV16/18-E7 and ER was not related do with clinicopathologic features of uterine cervical ad enocarcinoma, such as age, tumour size, clinical stages,lymphatic metastasis and so on （P=0.05）. The high expression of the Cyclin D1 was related with the clinical stage （X2 = 6. 234, P= 0. 013） and lymph node metastasis （X2 = 9. 038, P= 0. 003） in cervical adenocarcinoma, but not related with age, tumour size and the histology （P=0. 05）. The reslut ofWestern-Blot showed that the expression of Cyclin D1 were different in twenty--one cases of uterine cervical adenocarci noma in which the expression of HPV16/18-E7 and Cyelin D1 were positive. The expression of Cyclin D1 was no or low in seven cases of the control group. There were no statistics difference （Pcyain D1 = 0. 037）. The expression of pRb was no or low in the twenty-one cases of uterine cervical adenocareinoma. The expression of pRb was no difference in the control group （PpRb = 0. 002）. The expression of Cyclin D1 showed a positive correlation with HPV16/18-ET. 7 （x2 = 17.56, r= 0. 902 4,P=0. 042）. The expression of ER showed a positive correlation with HPV16/18-E7 （x2 =5. 431,r=0. 345 8, P=0.02）. The expression of Cyclin D1 showed a positive correlation with ER （x2=4. 713,r=0. 317 1,P=0.03）. CONCLUSIONS：The positive expression rates of Cyelin D1 is gradually increased, following the higher clinical stage of uterine cervical adenocarcinoma, and it is related with lymph node metastasis. The activated Cyclin D1/pRb signaling pathway may play an important role in the pathogenesis of cervical adenocarcinoma. ER is high expressed in the uterine cervical adeno carcinoma which are infected by HPV16/18. The,result indicates that estrogen may play an important role in the proces sion in which high-risk HPV16/18 may take part in.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2014年第4期256-259,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30970651)
国家重点实验室基金(SKLZZ200907)
