摘要
目的 建立腺病毒3型（HAdV-3）感染HEp-2细胞模型,评价六神丸混悬液对腺病毒感染HEp-2细胞病变的抑制作用,并进一步探讨六神丸在体外抗腺病毒的量效关系.方法 用乙醇回流法处理制备六神丸液.以MTT法计算药物的半数中毒剂量（TC50）.通过三种不同给药方式即预防给药、治疗给药及直接灭活给药方式进行体外实验,观察六神丸液对HAdV-3感染HEp-2细胞病变所起的作用,并对药物的量效关系进行分析.结果 六神丸液TC50值为40.48 μg/ml.预防、治疗及直接灭活给药方式均可减轻HAdV-3感染HEp-2细胞的CPE程度,其抗HAdV-3的半数有效浓度（IC50）分别为28.91μg/m1、19.28 μg/m1、8.61 μg/ml,治疗指数（TI）分别为1.4、2.1和4.7,六神丸液对HAdV-3感染HEp-2细胞CPE的抑制作用存在着一定的量效关系.结论 在预防、治疗及直接灭活给药方式下,六神丸均有一定的抗HAdv-3的作用,但直接灭活效果更明显.
Objective A model was established by this study to explore anti-adenovirus effect of different administration method of Chinese medicine Liushen pill on adenovirus type 3 （ADV-3） replication in HEp-2 cells.Metods Using ethanol refluxing to prepare Liushen pill suspension.TC50 was calculated by MTT assay.Cell culture was applied to observe the effect of adenovirus type 3（ADV-3） replication through prevention,treatment or direct inactivation of three different drug administration methods.Results Liushen pill suspension was found to be a potential inhibitor of Adv-3 in a concentration（TC50） of 40.48μg/ml in HEp-2 cell culture.The median inhibition concentration（IC50） was 19.28 μg/ml when drug was added after virus infection,and it was 8.61 μg/ml when drug was mixed with virus and added,and it was 28.91 μg/ml when drug was added before virus injection.The therapeutic index （TI） was 1.4,2.1,4.7 repectively.Conclusion The results showed Liushen pill suspension could inhibit the replication of ADV-3 and inactive ADV-3 in vitro.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2014年第1期47-49,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家级大学生创新创业训练计划
