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结肠癌组织与癌旁组织长链非编码RNA表达谱的筛选 被引量：10

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摘要 目的:应用高通量长链非编码RNA(long non-coding RNA,lncRNA)芯片检测结肠癌组织和癌旁组织差异表达的lncRNA,并对其进行初步分析。方法:分别提取6例结肠癌患者的癌组织和癌旁组织中的RNA,体外逆转录制备并标记为双链cDNA(ds-cDNA),与含有22 000条lncRNA的芯片进行杂交,计算机扫描并分析芯片荧光信号图像,筛选差异表达的lncRNA。结果:在结肠癌组织中差异表达的lncRNA达928条,其中在肿瘤组织中高表达的lncRNA 522条,低表达的lncRNA 406条(变化>2倍且P<0.05)。结论:结肠癌的发生过程中lncRNA的表达谱发生明显变化,lncRNA与结肠癌的发生密切相关。
作者 刘长 安公明 张胜龙 杨斌
机构地区 武钢大冶铁矿职工医院外科
出处 《实用医学杂志》 CAS 北大核心 2014年第5期781-782,共2页 The Journal of Practical Medicine
关键词 结肠肿瘤 长链非编码RNA 芯片
分类号 R735.35 [医药卫生—肿瘤]
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参考文献7

二级参考文献52

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共引文献16

同被引文献95

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实用医学杂志
2014年 第5期

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