摘要
目的 研究增殖细胞核抗原 (PCNA)在消化道肿瘤 ,尤其是胃癌中的表达及其临床意义。方法 应用免疫组化技术 ,对胃癌、食管癌、大肠癌和良性对照组织标本中PCNA的含量进行半定量检测 ,观察其与临床病理参数的关系及其临床意义。结果 (1)在良性对照中 ,增殖细胞核抗原标记指数 (PCNALI)为 (2 7 80± 12 2 0 ) ,明显低于各种肿瘤组 ,P <0 0 1。 (2 )在胃癌组、食管癌组和大肠癌组中 ,PCNALI分别为 (5 2 73± 12 2 5 )、(5 1 5 4± 10 2 4)和 (5 7 19± 7 78) ,相互之间经统计学处理 ,P >0 0 5。 (3)PCNA的表达与患者的性别、年龄、病变部位和大体分型无关 ,同时也与胃癌的组织学类型及其分化程度无关 (P >0 0 5 )。 (4 )PCNA的表达与患者的临床分期、浸润深度及淋巴结转移情况相关 ,P <0 0 5。结论PCNA的检测可作为判断肿瘤的恶性程度、预测淋巴结转移的趋势、指导制定合理的治疗方案和预后评估的有益指标。
Objective To study the expression of proliferating cell nuclear antigen (PCNA ) and its clinical significance in gastrointestinal carcinoma, especially in gastric cancer. Methods The expression of PCNA was semi-quantitatively investigated by SABC immunohisto-chemical technique in gastric cancer, esophageal cancer and colorectal cancer.Results 1.The PCNA Labeling index (LI) was (27.80±12.20) in benign group. The expression of PCNA was marked lower in benign group than that in malignant tumor group. ( P <0.01) 2. The PCNA LI was (2.73±12.25), (51.54±10.24), (57.19±7.78) in gastric cancer, esophageal cancer, and colorectal cancer respectively. There was no significant difference statistically ( P >0.05). 3. The expression of PCNA was correlated with the clinical stages, serosal infiltration and metastasis perigastric lymph nodes in gastric cancer ( P <0.05). Conclusion The detection of PCNA would be of benefit in reflecting the malignant degree, indicating lymph node metastasis, guiding treatment and evaluating prognosis tumor.
出处
《贵州医药》
CAS
2001年第2期113-115,共3页
Guizhou Medical Journal
