摘要
目的 探讨骨折愈合过程中骨形成蛋白 2 (BMP2 ) ,转化生长因子β1(TGFβ1) m RNA的表达与凋亡发生的相关性及意义 .方法 建立颌骨骨折模型 ,利用原位杂交方法检测骨折愈合的不同时期 BMP2和 TGFβ1m RNA的表达 ,同时采用 TUNEL 法检测细胞凋亡 ,并进行相关性分析 .结果 1骨折愈合早期 (5～ 11d)的间充质细胞、成骨细胞和软骨细胞内有 BMP2 m RNA表达 ,而 TGFβ1m RNA主要在骨折愈合后期 (11～ 14d)的成骨细胞和软骨细胞内表达 ;2细胞凋亡在骨折愈合的 5 d和 11d最多 ,以间充质细胞和软骨细胞为主 ;3BMP2 m RNA的表达与凋亡具有正相关 (r=0 .887,P<0 .0 1) ,TGFβ1m RNA的表达与凋亡无相关性 (r=0 .5 1,P>0 .0 5 ) .结论 骨折愈合过程伴随凋亡的发生 ,以控制细胞的数量和清除无用的细胞 。
AIM To elucidate the correlation of bone morphogenetic protein 2 (BMP2), transforming growth factor β1 (TGFβ1) mRNA expression and apoptosis during fracture healing and its significance. METHODS An experimental model of fracture was established to detect the expression of BMP2, TGFβ1 mRNA and apoptosis in the different stages of healing by using in situ hybridization and TUNEL methods. RESULTS ①BMP2 mRNA was expressed in mesenchymal cells, osteoblasts and chondrocytes on 5~11 d whereas TGFβ1 mRNA in osteoblasts and chondrocytes on 11~14 d post fracture; ②the apoptotic cells increased markedly on 5 d and 11 d, especially in mesenchymal cells and chondrocytes; and ③there was positive correlation between BMP2 mRNA expression and apoptosis ( r =0.887, P <0.01). TGFβ1 mRNA and apoptosis had no correlation ( r =0.51, P > 0.05 ). CONCLUSION Apoptosis is a normal concomitant of fracture healing serving the purposes of controling cell number and eliminating useless cells. BMP2 may participate directly in inducing the apoptotic process.
出处
《第四军医大学学报》
北大核心
2001年第1期37-39,共3页
Journal of the Fourth Military Medical University
关键词
骨形成蛋白
转化生长因子Β
骨折愈合
mRNA
细胞凋亡
bone morphogenetic protein
transforming growth factor β
fracture healing
apoptosis
in situ hybridization
TUNEL
