摘要
目的 探讨人骨形成蛋白 (rh BMP- 2 )对体内、外人脑胶质瘤 SHG44细胞周期和增殖的影响 .方法 体外培养人脑胶质瘤细胞 ,绘制 rh BMP- 2作用前、后的 SHG44细胞生长曲线 ,MTT法测定 rh BMP- 2对 SHG44细胞的增殖影响 ;以流式细胞仪、电镜和琼脂糖凝胶电泳分别检测其细胞周期、超微结构和 DNA片段改变 ;观察局部注射 rh BMP- 2对裸鼠皮下 SHG44胶质瘤增长的影响 .结果 rh BMP- 2可抑制SHG44细胞生长 ,其 G1期 DNA含量明显增加 ,S期含量减少 (P<0 .0 1) ;流式细胞仪检测有凋亡峰存在 (12 .3% ) ;电镜下见胞核染色质浓缩、边集和核碎裂 ,出现凋亡细胞 ;DNA电泳表现为梯状条带 (DNA ladder) .局部注射 rh BMP- 2后的SHG44胶质瘤生长减慢 ,体积缩小 (P <0 .0 1) .结论 rh BMP- 2可阻止 SHG44细胞从 G1期进入 S期 ,并能在体内、外抑制其增殖 。
AIM To investigate the effects of recombinant human bone mor phogenetic protein (rhBMP 2) on the cellcycle and proliferation of human glioma cells (SHG44) in vivo and vitro . METHODS The human SHG44 glioma cells were cultured in vitro . The growth curve of SHG44 cells with or without rhBMP 2 was plotted. The proliferation effect of rhBMP 2 on SHG44 cells with rhBMP 2 was determined by MTT method. The cell cycle, ultrastructure and DNA fragments of SHG44 cells were detected by flow cytometry, electron microscope and gel electrophoresis agarose respectively. The growth state of SHG44 glioma growing under the skin of nude mice was observed with the local injection of rhBMP 2. RESULTS The rhBMP 2 could inhibit the growth of SHG44 cell in vitro . The DNA content of SHG44 increased in G1 cycle, and decreased in S cycle obviously ( P <0.01). An apoptosis spike (12.3%)was found by FCM assay. The nucleolus chromatin aggregation, bound of aggregation, caryorrhexis and apoptosis cells were observed under the electron microscope. The DNA electrophoresis showed a ladder strap(DNA ladder). SHG44 glioma growth in mice was relatively slower and its volume was smaller with local injection of rhBMP 2 than those without rhBMP 2 injection ( P <0.01). CONCLUSION SHG44 cells could be arrested at G1/S stage and the cell growth could be significantly suppressed by rhBMP 2. The prosess might be carried out through rhBMP 2 induced apoptosis in human glioma cells.
出处
《第四军医大学学报》
2000年第9期1086-1089,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金! (39970 85 4)
全军"九五"基金!(97-M-14 1)
