摘要
糖苷生物碱(steroidal glycoalkaloid,SGAs)含量密切关系马铃薯块茎的食用品质和加工品质。本文利用GGE-biplot双标图分析了红光处理6、12、24 h后,5个马铃薯品种块茎中调控糖苷生物碱合成途径7个主要基因的表达情况。pvs1、sgt1和sgt3这3个基因的表达量均远高于其他4个基因的表达。在所测基因型中,sgt3的表达量不仅高而且还相对于pvs1和sgt1来说比较稳定,而在不同处理时间之间差异较大,稳定性较差。在12与24 h处理后所有基因表达的趋势比较接近,但是除了pvs1基因的表达量,其他6个基因的表达量均低于6 h处理的。从基因型的角度分析,所有基因在野生种HA和当地栽培种ZH-3中表达量较高而且表达趋势比较一致。通过比较调控糖苷生物碱合成途径的不同阶段的基因的表达量,发现不同阶段关键基因的表达量在基因型之间存在显著差异。所以,通过GGE-biplot分析,结果展示了7个基因在不同基因型马铃薯块茎的表达趋势,而且也根据基因表达量直观的展示了处理时间与各基因型之间的聚类与关系,为将来进一步分析不同光质在基因水平调控马铃薯糖苷生物碱的生物合成提供了重要参考。
Steroidal glycoalkaloids （SGAs） are a family of secondary metabolites that closely associate with the eating and processing qualities of potato tuber. In the present study, GGE-Biplot was used to analyze the transcriptional gene expression of seven genes that involved in the SGAs biosynthesis pathway. The microtubers of five varieties （genotypes） were incubated for 6, 12 and 24 h under red light, and the expression of seven genes were measured with RT-PCR and further analyzed via GGE-Biplot. The data indicated that the expression levels of pvs1, sgt1 and sgt3 were significantly higher among all tested genes. In addition, the transcriptional abandon of sgt3 was high and stable in all tested genotypes, but showed less stability among the light treatment durations. The very similar expression patterns of seven genes were observed for both 12 and 24 h treatments, while expressions of all except pvs1 were relatively lower after 6 h treatment. Among all tested genotypes, similar expression patterns of all genes were observed for HA and Zh-3. The genes that mediated different stages of SGAs biosynthesis also showed great variations among genotypes. Therefore, the results demonstrated that GGE-Biplot is a very useful tool to analyze and display the differential expression profile of seven key genes in potato tubers under red light illumination. According to the quantitative data, the results also presented the relationship among treatments and genotypes. The outcome of this study thus provides valuable reference for future study on SGAs biosynthesis, as well as the potato tuber quality improvement via manipulating the SGAs biosynthesis.
出处
《植物研究》
CAS
CSCD
北大核心
2014年第2期238-244,281,共8页
Bulletin of Botanical Research
基金
国家科技支撑计划(2012BAD06B03)
甘肃省科技重大专项计划(1102NKDA025)
国家马铃薯产业技术体系
甘肃省干旱生境作物学重点实验室开放基金(GSCS-2010-16)
关键词
马铃薯
双标图
糖苷生物碱
基因表达
Potato
GGE-Biplot
steroidal glycoalkaloids
gene expression
