摘要
目的探讨低分子肝素(LMWH)、N-乙酰半胱氨酸(NAC)对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠气道重塑的影响。方法将40只6~8周鼠龄按雌雄各半的SD大鼠分为5组,即健康对照组(C组),熏香烟及脂多糖(LPS)方法制备的COPD模型组(M组),以及低分子肝素(LMWH)注射组(L组)和N-乙酰半胱氨酸(NAC)灌胃组(N组)及前二者同时干预组(LN组);光镜下观察大鼠病理学改变;以图像分析系统测量其支气管壁厚度;偏振光显微镜下观察各组大鼠支气管黏膜下胶原天狼猩红染色的气道胶原沉积。结果①模型制备:本研究制备的COPD模型大鼠支气管、肺病理改变与人类COPD变化基本一致。②支气管壁厚度:与对照组比较,M组、L组、N组和LN组管壁厚度明显增加(P<0.01);而与M组比较,L组、LN组管壁厚度均减少,且以LN组减少最为明显(P<0.01),N组亦有减少但差异无统计学意义(P>0.05)。③胶原沉积:与对照组比较,各组主要呈现Ⅰ型胶原沉积,L组和N组胶原减少,LN组胶原减少更趋明显。结论①较短时间的熏香烟+灌注LPS是建立COPD大鼠模型的简单易行的方法;②LMWH、NAC干预尤其是联合应用可通过不同作用机制减轻COPD发病过程中气道壁厚度及气道胶原沉积,进而减轻COPD气道重塑。
Objective To investigate the effects of low molecular weight heparin （LMWH） and N- acetylcysteine （NAC） intervention on airway remodeling of chronic obstructive pulmonary disease （COPD） rats. Methods A total of 40 SPF-SD rats aged 6-8 weeks were equally divided into healthy control group （group C）, COPD model （induced through cigarette smoke inhalation and intratracbeal lipopolysaccharide infusion） group （group M）, LMWH （subcutaneous injection of LMWH） intervention group （group L）, NAC intervention （intragastric infusion of NAC） group （group N）, and LMWH＋NAC intervention group （group LN）. We observed the pathological changes under light microscope, measured the bronchial wall depth by image analysis system, and checked airway submucosal collagen deposition using sirius red stain under the polarized microscope. Results （i） COPD rat models had similar pathological changes to human COPD. （2） The depth of bronchial wall increased in groups M, L, N and LN compared with group C （P〈0.01）. It decreased in groups L and LN as compared with group M, and the decrease was most significant in group LN （P〈0.01）. But there was no significant decrease of airway wall depth in group N （P^0.05）. @ There was deposition of type I collagen in all the groups, the decrease of collagen was more significant in group LN than in groups L and N. Conclusion @ COPD rat model can be easily constructed by smoke inhalation and intratracheal lipopolysaccharide （LPS） infusion in shorter duration; （2） LMWH and NAC intervention independently or in-combination （in particular） can reduce the depth of bronchial wall and collagen deposition of COPD, thus decreasing airway remodeling in COPD.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2014年第2期196-199,217,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
慢性阻塞性肺疾病
低分子肝素
N-乙酰半胱氨酸
支气管壁厚度
胶原沉积
气道重塑
chronic obstructive pulmonary disease （COPD）
low molecular weight heparin （LMWH）
N-ace- tylcysteine （NAC）
bronchial wall depth
collagen deposition
airway remodeling
