摘要
在真菌培养过程中,对其个体形态与群体(菌落)形态进行实时观察与鉴定是很必要的。本文利用半培养基培养法结合显微操作技术,对丝状真菌个体与群体进行形态学观察。结果表明,该方法无需染色与制片,不破坏菌丝正常生长状态,可实时进行形态学检测,对多个菌种在自然生长状态下的菌丝与菌落特征进行观察,操作简单、方便、快捷,从而降低了成本及工作量。
It is important to observe and identify the morphology of individual form and group（colony） during the cultivation of fungi.In this study,half medium combined with micromanipulation technique was used to observe the morphological changes in individual and group of filamentous fungi.The results showed that this novel method could be used to make real-time measurements of the morphology of filamentous fungi without dyeing and slicing,and does not affect the normal growth of mycelia.In addition,it can be applied to many strains for observing the mycelium undergoing natural growth and the colony characteristics simultaneously.Overall,in this experiment,a simple and rapid method is designed for the observation of individual and colony morphology of filamentous fungi,which needs a low cost and workload.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第2期229-232,共4页
Plant Physiology Journal
基金
国家基础科学人才培养基金(J1210053)
国家自然科学基金(31101171)
东北林业大学高等学校教学改革及细胞生物学重点课程建设项目
关键词
丝状真菌
形态观察
鉴定
filamentous fungi
morphological observation
identification
