摘要
研究目的是筛选高产漆酶的白腐菌株.通过愈创木酚培养基和鞣酸培养基对白腐菌所产漆酶的显色作用来筛选白腐真菌,并分析了所分离出的白腐菌的产漆酶能力.运用此种方法,成功获得5株高产漆酶的白腐真菌,其中一株白腐真菌所产漆酶量达到了211.7IU/mL,比普通白腐菌漆酶产量高出1.12倍.最后,用光学显微镜对所分离出的白腐真菌进行形态特征观察,和文献报道的白腐菌形态特征一致.
The purpose of this study is to screen the high yield and laccase white rot strains. Through the method to screening white-rot fungus that the guaiacol medium and tannic acid medium dialogue rot fungus produce of laccase color effect, and analyzed the separated from white-rot fungi yield laccase ability. By this method, successfully obtained five the high yield laccase white rot fungi, laccase produced by a white rot fungus reached 211.7 IU/mL,1.12 times higher than the ordinary white rot fungi laccase production. Last using the light mi- croscopy on the separated white rot fungal to morphological observation and found the mor- phological features are same with reported in the literature.
出处
《陕西科技大学学报（自然科学版）》
2012年第6期79-83,共5页
Journal of Shaanxi University of Science & Technology
基金
陕西省科学技术研究发展计划社会发展科技攻关项目(2010K01-200)
陕西省自然科学基金项目(2010JQ4020)
陕西省教育厅科技计划项目(12JK1023)
国家自然科学基金项目(20906058)
陕西科技大学学术骨干培育项目(XSG2010009)
关键词
白腐菌
漆酶
分离
white rot fungi
laccase
separation
