摘要
目的 观察凋亡相关基因白介素 - 1β转化酶 (ICE)、Bcl- 2在脑缺血后的表达情况以及丹参(RSM )对其影响。方法 健康沙土鼠 6 3只 ,随机分成三组 :假手术组、对照组和丹参组。采用沙土鼠前脑缺血再灌注模型 ,在沙土鼠脑缺血再灌注后的 2h、6h、12h、1d、3d、5d及 7d分别处死沙土鼠 ,将脑组织进行免疫组织化学染色观察。结果 ICE的蛋白表达主要在海马CA1区、CA4 区及皮质 ,免疫阳性细胞呈褐色 ,第 3天达高峰 ,第 5天后减少 ;丹参组ICE的蛋白表达明显减少 (P <0 .0 1)。Bcl- 2的蛋白表达主要在大脑皮层、纹状体、丘脑及海马等广泛区域 ,并于 6h达高峰 ,其后减少 ;丹参组Bcl - 2的蛋白表达明显增加 ,特别是在海马CA1区及大脑皮层区 (P <0 .0 1)。结论 ICE在调节脑缺血中可能发挥重要作用 ;Bcl- 2主要通过抑制细胞凋亡的早期环节而发挥作用 ;丹参能下调脑缺血后的ICE表达、上调脑缺血后的Bcl- 2表达进而发挥其神经保护作用。
Objective To study the expression of ICE、Bcl-2 after ischemia and reperfusion and to study the effects of Radix Salviae Miltiorrhizae(RSM) on them.Methods 63 gerbils were divided into three groups(sham-operated group,control group and RSM group), and decapitation were operated on gerbils at 2h、6h、12h、1d、3d、5d、7d after ischemia and reperfusion. Immediately, using immunohistochemical technique, we observed the expression of Interleukin-1 β converting enzyme(ICE),Bcl-2 and the effects of RSM on them.Results Expression of ICE were observed markedly in CA 1,CA 4 and cortex and the neurons were in brown with the peak at 3rd day and decreasing at 5th day after ischemia and reperfusion.Bcl-2 were markedly induced in cortex,stria body,thalamencephalon and hippocampus with the peak at 6h. We also found that expression of ICE were down-regulated and that of Bcl-2 were up-regulated in RSM group( P <0.01). Conclusion Our results indicate that ICE may play an important role in regulating ischemic reperfusion, Bcl-2 may restrain apoptosis at the early phase and RSM may ameliorat ischemic damage by reducing the apoptosis through regulating ICE and Bcl-2.
出处
《卒中与神经疾病》
2001年第1期26-28,共3页
Stroke and Nervous Diseases
