摘要
目的探讨基因工程人血管内皮抑制素（ｅｎｄｏｓｔａｔｉｎ）抑制黑素瘤在小鼠体内生长和转移的作用及其作用机制。方法接种黑素瘤细胞悬液（２ × １０６／鼠）于小鼠皮下，成瘤后给予血管内皮抑制素（８ ｍｇ／ｋｇ· ｄ），每天 １次，共２１次。给药期间观察荷瘤小鼠的饮食情况、体重变化，并测量肿瘤的大小。于第２６天处死，称皮下肿瘤的重量，并取脑、肺、肝、脾和肾切片做病理学检查。结果血管内皮抑制素治疗组的曲线下面积明显小于肿瘤对照组（Ｐ＜０．０１）。病理切片检查显示，治疗组肿瘤有大面积的坏死，瘤周毛细血管消失。结论血管内皮抑制素对小鼠黑素瘤的生长、转移，以及新生毛细血管的形成具有明显地抑制作用。
Aim To explore inhibitory effects and mechanism of engineering endostatin on growth and metastasis of melanoma cells in the. methods Melanome cells(2 × 106/mouse) were inoculaled sabcutaneously to C57BL/6 mice. After tumorigenesis, endostatin(8mg/kg.d) was administrated to tumor-bearing mice, once a day , twenty-one in all. Dietetic state and weight change of the tumor-bearing mice were observed and tumorous sige was measured during administration of endostatin. On 26thday, the tumor-bearing mice were sacrificed, subcutaneously tumorous weight was weighed and brain. lung, liver, spleen and kidney were excised and sections were made to supply the pathological examination. Results Area under curve in the endostatin-treated group was obviously less than that in tamor control group(P< 0. 01 ). Pathological study revealed that lavge areal necrosis arose in tumor and newborn cappillaries around the tumor disapeared. Conclusion Endostatin possosses strongly inhibitory effects on growth and metastasis of mouse melanoma and formation of newborn capillaries around tumor.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第1期63-64,共2页
Chinese Journal of Cellular and Molecular Immunology
