摘要
目的：观察针刀干预对颈椎病兔劳损颈肌细胞凋亡的影响，揭示针刀治疗颈椎病可能的疗效机制。方法：6月龄新西兰兔随机分为空白组、模型组、针刀组、电针组，每组各6只，采用长期低头位方法制备颈椎病模型。造模后针刀组于斜方肌起点、胸锁乳突肌乳突附着点、颈椎关节突及局部硬结或条索状物，每次选2～3个点定为针刀进针部位，针刀干预每周1次，共3次；电针组取双侧“天柱”“颈百劳”“大杼”，毫针针刺，同侧“天柱”“大杼”穴接韩氏穴位神经刺激仪，每次治疗20min，隔天1次，共3周。干预结束1周后取颈肌行TUNEL检测和Westernblot检测Bcl-2、gax蛋白表达水平。结果i颈肌TUNEL阳性细胞核计数模型组较空白组明显升高（P〈0．01），针刀组较模型组、电针组明显降低（P〈0．01）。Westernblot结果显示，各组Bcl-2蛋白表达水平差异无统计学意义（P〉0．05）；模型组Bax蛋白表达水平较空白组明显升高（P〈0．01），针刀组Bax蛋白表达水平较模型组降低（P〈0．05）；模型组Bcl-2／Bax蛋白比值较空白组降低（P〈0．05），针刀组Bcl-2／Bax蛋白比值较模型组、电针组升高（P〈0．05）。电针组各项指标与模型组比较差异均无统计学意义（P〉0．05）。结论：针刀干预颈椎病可降低颈肌细胞凋亡，这可能是针刀治疗颈椎病的疗效机制之一。
Objective To observe the effect of acupotomy therapy on cervicomuscular apoptosis and apoptosis regula- tor Bax protein expression in cervical spondylosis （CS） rabbits so as to investigate its mechanisms underlying improvement of CS. Methods Twenty-four New Zealand rabbits were randomly divided into normal control, model, acupotomy and electroacupunc- ture （EA） groups, with 6 rabbits in each group. The CS model was made by forced head-bowing for 5 hours in a restrained cham- ber, once daily for 12 weeks. Acupotomy was performed at the starting point of trapezius, the mastoid process attaching point of sternocleidomastoid, the cerverical vertebrae joint process or the local induration or cord-like mass （2 or 3 points of them were used as the needle-knife entering points）, once a week for 3 weeks. For animals of the EA group, EA （2 Hz/100 Hz, 2 mA） was applied to bilateral “Tianzhu” （BL 10）, “Jingbailao” （EX-HN 15）, “Dazhu” （BL 11） for 20 min, once daily and 3 times a week for 3 weeks. The number of apoptotic cells in the cervical muscle was observed by light microscope after TUNEL staining and mus- cular Bcl-2 and Bax protein expression was detected by Western blot. Results In comparison with the control group, the num- ber of cervicomuscular apoptotic cells, and the expression level of cervicomuscular Bax protein were significantly increased, and the Bcl-2/Bax was obviously decreased in the model group （P〈0.01, P〈0.05）. Compared to the model group, the number of apoptotic cells and the expression level of muscular Bax protein were notably decreased in the acupotomy group （ P〈0.01, P〈0.05）, while the ratio of Bcl-2 and Bax was apparently increased in the acupotomy group （P〈0.05）. The effects of acupoto- my were significantly superior to those of EA in lowering apoptotic cell number and in up-regulating Bcl-2/Bax （P〈0.01, P〈0.05）. No significant differences were found between the EA and model groups in the apoptotic cell number and among the four groups in Bcl-2 protein expression levels （P〉0.05）. Conclusion Acupotomy therapy can reduce cervicomuscular cellular apoptosis and Bax protein expression in CS rabbits, which may be one of its mechanism underlying improving CS.
出处
《针刺研究》
CAS
CSCD
北大核心
2014年第1期68-72,共5页
Acupuncture Research
基金
北京中医药大学自主选题(No.2011-JYBZZ-XS 102)
江西中医学院校级课题(No.2012BS 1002)
