摘要
目的:观察电针对溃疡性结肠炎(UC)大鼠肠道菌群的影响,从肠道微生态的角度探讨电针治疗UC的机制。方法:39只健康雄性SD大鼠随机分为空白组(13只)和UC造模组(26只),造模组饮用4%的葡聚糖硫酸钠(DSS)溶液7d复制UC模型后随机分为模型组和电针组。电针"天枢""足三里""上巨虚"穴,每日1次,每次10min,治疗5d。观察实验过程中大鼠疾病活动指数的变化;收集各组大鼠粪便排泄物,用多聚酶链反应-变性梯度凝胶电泳法分析肠道菌群的多样性、相似性及丰度值,对优势条带进行回收测序,采用Quantity one等软件对所得结果图谱进行分析,对优势条带进行菌种鉴定及含量分析。结果:模型大鼠肠道菌群的丰度值及多样性指数明显降低,疾病活动指数明显增高(P<0.05,P<0.01)。电针能降低UC模型大鼠疾病活动指数,提高肠道菌群的丰度值及多样性指数(P<0.05,P<0.01);菌种鉴定表明电针能提高UC模型大鼠肠道菌群中乳酸杆菌(Lactobacillus sp.)和毛螺科菌(Lachnospiraceae bacterium)含量,降低双酶梭菌(Clostridium bifermentans)的含量(P<0.05)。结论:电针能明显改善UC模型大鼠肠道菌群的多样性以及有益菌群的含量,对UC模型大鼠肠道微生态发挥的保护作用很可能是电针治疗UC的机制之一。
Objective To observe the effect of electroacupuncture （EA） on enteric microbial populations in ulcerative colitis （UC） model rats, so as to study its mechanisms underlying improving UC. Methods Thirty-nine male SD rats were ran- domly divided into control group, model group and EA group （n = 13 in each group）. The UC model was established by intragstric perfusion of 4 % dextran sodiumaqueous solution （DSS）, once daily for 7 days. EA （15- 25 Hz, 0.1 mA） was applied to bilateral “Tianshu” （ST 25）, “Zusanli” （ST 36） and “Shangjuxu” （ST 37） for 10 min, once daily for 5 days. Disease Activity Index （DAD including body weight and stool blood state was used to evaluate the animals’ conditions. The rats＇ excrement was collected to detect the abundance and diversity of bacterial populations by polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）. Results In comparison with the control group, the animals＇ DAI score and enteric Clostridium bifermentans con- tent were significantly increased （P〈0.05）, and the abundance and diversity of bacterial populations, and contents of enteric Lachnospiraceae bacterium and Lactobacillus sp. were considerably down-regulated in the model group （P〈0. 05, P〈0. 01）. Compared with the model group, the DAI score and enteric Clostridium bifermentans content were significantly lowered, and the abundance and diversity of bacterial populations, and contents of enteric Lachnospiraceae bacterium and Lactobacillus sp. were notably increased in the EA group （P〈0.05, P〈0. 01）. Conclusion EA can improve DAI and the abundance and diversity of enteric bacterial microbiota in UC rats, which may be related to its protective effect on enteric microecology in UC.
出处
《针刺研究》
CAS
CSCD
北大核心
2014年第1期27-34,共8页
Acupuncture Research
基金
国家自然科学基金(No.30901933
81373737)
四川省教育厅重点项目(No.11ZA 055)
成都中医药大学校基金(No.ZRYB 201101
ZRYY 201213)
关键词
溃疡性结肠炎
电针干预
肠道微生态
多聚酶链反应-变性梯度凝胶电泳
Ulcerative colitis
Electroacupuncture interventions Enteric microecology
Polymerase chain reaction-denaturing gradient gel electrophoresis
