摘要
目的探讨白藜芦醇(resveratrol,RSV)对阿霉素(doxorubicine,DOX)引起的心肌细胞损伤的保护作用及相应机制。方法应用DOX,RSV,SIRT1 siRNA处理H9c2细胞,对照组(CON)不做任何处理。MTT比色法检测H9c2细胞活力,流式细胞术检测凋亡率,Western免疫印迹检测SIRT1蛋白表达,荧光显微镜下观察转染GFP的细胞,并随机选取视野计算转染率。结果 DOX降低细胞活力,诱导细胞凋亡,呈现时间、剂量依赖性。RSV时间、剂量依赖性上调SIRT1蛋白表达。RSV预处理能明显降低DOX对H9c2细胞的损伤。然而,SIRT1 siRNA预处理则加重DOX引起的H9c2细胞损伤。这种细胞损伤未能被RSV预处理所改善。结论 RSV可减轻DOX引起的H9c2细胞损伤,这种保护作用可能是通过上调SIRT1来实现的。
Aim To observe the protective effect of resveratrol on doxorubicine （DOX）-induced injury in H9c2 cardiac ceils and the underlying mechanism. Methods H9c2 cells were treated with DOX to estab- lish a model of cardiomyocyte injury. Resveratrol was applied before exposure to DOX to activate SIRT1, and SIRT1 siRNA was used to inhibit SIRT1. MTI＂ was used to detect the viability of H9c2 cells. Flow cytome- try was used to determine the apoptosis of H9e2 cells. The expression of SIRT1 was detected by Western blot. H9c2 cells transfected with GFP were observed under a fluorescence microscope, and the transfection efficien- cy was calculated in a randomly-selected view. Results DOX decreased cell viability and induced apoptosis in time-and dose-dependent pattern. In contrast,SIRT1 protein was significantly upregulated by RSV also in a time- and dose-dependent way. Pre-treatment with RSV effectively reduced injury of H9c2 ceils induced by DOX. Pre-treatment with SIRT1 siRNA aggravated the injury of H9c2 cells induced by DOX, which could not be alleviated by RSV treatment. Conclusion RSV can significantly protect H9c2 cells against DOX in-duced injury possibly by upregulating the expression of SIRT1 protein.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第2期220-224,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金青年基金资助项目(No 81100099)
国家自然科学基金面上项目资助(No 81070110)
上海市科委国际科技合作基金项目(No 11410701900)
