摘要
目的探讨转录因子SP1的小干扰RNA(SP1-siRNA)对胶质瘤细胞系U251凋亡与增殖的抑制作用。方法将SP1-siRNA转染至U251细胞中,分别用Western blot检测siSP1的转染效率,流式细胞术检测U251细胞转染siSP1后的凋亡,CCK-8法检测U251转染siSP1后的增殖,Western blot检测U251转染siSP1后SP1下游的生存素蛋白水平,半胱氨酸天冬氨酸蛋白酶3(Caspase-3)活性检测试剂盒检测U251转染siSP1后Caspase-3活性。结果 U251细胞系转染siSP1后,转染组凋亡率高于空白对照组和阴性对照组(P<0.05),转染组增殖低于空白对照组和阴性对照组(P<0.05),转染组生存素蛋白表达水平低于对照组(P<0.05),转染组Caspase-3活性高于对照组(P<0.05)。结论转录因子SP1-siRNA可以有效抑制胶质瘤U251细胞系的凋亡与增殖。
Objective To explore the inhibitory effect of transcription factor specificity protein I（SP1）-siRNA on the apoptosis and proliferation of glioblastoma U251 cells. Methods SPI-siRNA was transfected into U251 cells. The transfection efficacy of SPI-siRNA was examined with Western blot. After SPI-siRNA transfection, the apoptosis of U251 cells was detected by flow cytometry, proliferation by CCK-8, servivin expression by Westem-blot, and Caspase-3 activity by Caspase-3 activity test box. Results Compared to the U251 cells in blank and negative .control groups, the apoptosis rate was higher and proliferation was lower in the U251 ceils transfected with SPI-siRNA （P〈0. 05）. Compared to the U251 cells in blank control group, the expression of survivin was downregulated and Caspase-3 activity was enhanced in SPl-siRNA-transfected U251 cells（P〈0. 05）. Conclusion The SPI-siRNA can effectively suppress the apoptosis and proliferation of glioblastoma U251 cells.
出处
《江苏医药》
CAS
北大核心
2014年第4期373-376,共4页
Jiangsu Medical Journal
基金
国家自然科学基金(81172389
81101901)
江苏省自然科学基金(BK2010580
BK2011847)
江苏省科教兴卫工程医学重点学科(XK201117)
