摘要
目的以气相色谱．质谱法（GC．MS）测定地锦喷雾剂中丁香酚的含量。方法色谱柱为DB．5弹性石英毛细管柱（30m×0．25mm，0．25μm），采用程序升温法测定样品中丁香酚的含量：初始温度60℃保持1min，以10℃／min升温至200℃保持5min，再以15℃／min升温至250℃保持5min。质谱条件：离子源：电子能量70ev。在离子采集模式下，选取离子为164、149、77对丁香酚进行检测。结果丁香酚的浓度线性范围为0．116～1．16mg／ml（r=0．9994），平均回收率为97．84％（RSD=0．47％）。结论本方法快速、灵敏、准确，重复性好，可用于控制地锦喷雾剂中丁香酚的含量。
Objective To determine the eugenol in Dijin spray by GC-MS. Methods The chromato-graphic column was DB-5 fused silica capillary column. Eugenol was determined by program temperature risen method： the initial temperature was 60 ℃for 1 rnin, then raised to 200 ℃ at the rate of 10 ℃/min and kept for 5 min, and at last raised to 250 ℃ at 15 ℃/min and kept for 5 min. Electron energy was 70ev. The ions of 164, 149, and 77 were selected for eugenol detection. Results The calibration curve of eugenol was linear in the range of0.116-1.16 mg/ml （r=0.999 4） with the average recovery of 97.84% （RSD= 0.47%）. Conclusion The method was quick, accurate and repeatable. It was suitable for the quality control of Dijin spray.
出处
《国际中医中药杂志》
2014年第3期237-239,共3页
International Journal of Traditional Chinese Medicine
基金
济南军区后勤科研计划（项目编号：CJN12L078）
