摘要
目的了解2011年1月-2012年12月上海市普陀区手足口病病原学特征,为手足口病的防控提供实验室依据。方法采集432例手足口疑似病例的咽拭子、疱疹液,粪便标本共723份,用实时荧光定量PCR方法进行肠道病毒通用型(EV)、肠道病毒71型(EV71)、柯萨奇病毒A组16型(CoxA16)的核酸检测。结果 723份手足口病例标本肠道通用型阳性394份(54.50%),肠道病毒71型(EV71)阳性160份(22.13%),柯萨奇病毒A组16型(CoxA16)阳性165份(22.82%)。结论上海市普陀区手足口病不同时期病原体呈现交替流行,患者年龄分布和时间分布特征明显,对手足口病原体的流行病学分析有助于做好手足口病的防控工作。
Objective To investigate the etiological characteristics of hand foot and mouth disease （HFMD） from January 2011 to December 2012 in Putuo district of Shanghai, so as to provide the laboratory basis for prevention and control of HFMD. Methods Real - time fluorescent quantitative PCR technique was used for nucleic acid detection of universal enteroviruses （EV）, enterovirus 71 （EV71） , coxsackievirus A16 （CoxA16） in 723 samples of throat swabs, herpes fluid and stools from patients with suspected HFMD. Results There were 394 universal enteroviruses positive samples （54.50%）, 160 EV71 posi- tive samples（22.13% ） and 165 CoxA16 positive samples（22.82% ） in the 723 HFMD samples. Conclusion HFMD patho- gens showed ahemative epidemic characteristics at different times and distinct distribution features of age and time among pa- tients in Putuo district, Shanghai. The epidemiology analysis of HFMD pathogens is helpful to prevent and control the HFMD.
出处
《中国卫生检验杂志》
北大核心
2014年第3期413-415,共3页
Chinese Journal of Health Laboratory Technology
