摘要
目的:电化学法检测原位收集获得的MCF-7细胞电化学信号,建立操作简单,快速、敏感、价廉、细胞用量少的原位细胞电化学检测方法。方法:采用循环伏安法研究原位细胞收集法对电化学信号的影响。结果:采用电化学法检测原位收集获得的MCF-7细胞质,其电化学信号高于传统收集的细胞质电化学信号。结论:原位细胞收集缩短了检测前细胞处理的准备过程,提高了细胞电化学响应强度。
Objective： Studying the electrochemical signal of MCF-7 cells obtained by in-situ electro- chemical method and establishing simple, rapid, sensitive and inexpensive in-situ cells electrochemical detec- tion. Methods： Cyclic Voltammetry method was used for detection the electrochemical signals of cells collection using in-situ method. Results： Electrochemical detection method results display that the peak current value of cells of in-situ collection is higher than that of conventional method. Conclusion： The In-situ ceils collection method shorten the process of preparing cells before testing and improve electrochemical response strength of cells.
出处
《黑龙江医药科学》
2014年第1期91-92,共2页
Heilongjiang Medicine and Pharmacy
基金
黑龙江省自然科学基金项目
编号:B201005
黑龙江省大学生创新训练计划项目
编号:2012XJ029
黑龙江省教育厅项目
编号:12511542
