摘要
目的 探讨化疗对肝癌患者T淋巴细胞内的白细胞介素 2 (IL 2 )、干扰素γ(IFN γ)的影响。方法 应用单克隆抗体和流式细胞术 (FCM )分析 31例肝癌患者化疗前后T淋巴细胞内IL 2、IFN γ水平的变化。结果 肝癌患者化疗后T淋巴细跑内IL 2和IFN γ均较化疗前降低 (P <0 .0 5 ) ;静脉化疗组与介入化疗组化疗前后T淋巴细胞内IL 2及IFN γ改变差值均无显著性差异 (P >0 .0 5 ) ;不同临床分期化疗前后IL 2及IFN γ改变差值均无显著性差异 (P >0 .0 5 )。结论 化疗药物可通过降低T淋巴细胞内的IL 2、IFN γ水平而影响细胞免疫功能 ,导致机体免疫功能降低。
Objective To investigate the influence of chemotherapy on intracellular IL 2 and IFN γ levels of T lymphocytes in patients with liver cell carcinoma.Methods 31 patients underwent chemotherapy for liver cell carcinoma,2 ml of venous blood was obtained before chemotherapy and on the 7th day after chemotherapy.The intracellular IL 2 and IFN γ levels of T lymphocytes were analysed by flow cytometry and monoclonal antibodies.Results The expression rates of intracellular IL 2 and IFN γ in the T lymphocytes were lower in post chemotherapy than in pre chemotherapy( P <0.05),and the changes of intracellular IL 2 and IFN γ were not different as different chemotherapy methods be used( P >0.05),and the changes were not different among the different clinical status( P >0.05).Conclusion The chemotherapy can effect cell immune functions by decreasing intracellular IL 2 and IFN γ in patients with liver cell carcinoma,and depress their immune function.
出处
《实用癌症杂志》
2001年第1期76-77,86,共3页
The Practical Journal of Cancer
