摘要
目的 :探讨分析结肠癌患者外周血淋巴细胞与结肠癌组织内谷胱甘肽硫转移酶 (GST)活性变化及二者的相互关系 ,评估结肠癌患者外周血淋巴细胞内GST活性变化在结肠癌发生发展过程中的临床价值。方法 :应用1-氯 - 2 ,4-二硝基苯为底物的比色分析法 (34 0nm) ,对 31例结肠癌患者外周血淋巴细胞和癌组织内GST活性变化及二者之间的关系进行了分析。结果 :1)结肠癌患者较正常人淋巴细胞内GST活性明显升高 ,分别为 2 83±2 0 4和 96± 14 3(P <0 0 1)。 2 )结肠癌组织较正常临近组织内GST活性明显增高 ,分别为 14 7± 2 2 9和 37±10 8(P <0 0 1)。 3)结肠癌患者外周血淋巴细胞内与癌组织内GST活性变化具有良好的相关性 (r =0 80、P <0 0 1)。 4)正常人及结肠癌患者淋巴细胞内GST活性变化相对稳定 ,分别在 89 3～ 110 6和 2 5 9 9～ 310 4之间波动。 5 )未转移结肠癌患者外周血淋巴细胞内GST活性在手术一周后基本恢复正常。 6 )结肠癌患者外周血淋巴细胞内GST活性变化与性别、年龄及肿瘤的临床分期无关。结论 :这些研究提示作为肿瘤标记物的GST ,其在外周血淋巴细胞内的活性变化相对稳定 ,并且与结肠癌组织内GST活性变化成正相关。
Objective: To study the changes of glutatheione sulfural transferase (GST) activation in peripheral blood and cancer tissue, and to evaluate their clinical value in colorectal cancer Methods: GST activation both in blood and cancer tissue were determined by 1-chlorine-2, 4-dinitrobenzene colourmeterology Results: 1) GST activation increased in cancer tissue more significantly than that in its surrounding tissue (14 7±22 9, 37±10 8, P< 0 01) 2) Lymphocytic GST activation in cancer patients were higher than that in normal individual (283±20 4, 96±14 3, P< 0 01) 3) GST activation in cancer tissue well correlated with that in blood lymphocytes (r=0 80, P< 0 01) 4) Lymphocytic activation was relatively stable both in normal control and colorectal cancer patients 5) Peripheral lymphocytic GST returned to normal in one week after removal of cancer 6) Lymphocytic GST in cancer patients had no correlation with their sex,age and clinical staging Conclusion: Peripheral blood lymphocytic GST is a useful index for discovering, screening and estimating recrudescence of colorectal cancer
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2000年第5期356-358,共3页
Chinese Journal of Clinical Oncology
