摘要
研究了不同质量浓度(4、8、12、16和20 mg·L-1)水稻(Oryza sativa)和再力花(Thalia dealbata)秸秆水浸提液对水绵(Spirogyra sp.)生长的抑制作用。结果显示,试验96 h后各处理组浸提液对水绵的生长均有抑制作用,随着浸提液浓度的增大,水绵细胞叶绿素a含量逐渐降低,显示出明显的剂量-效应关系。再力花ρ(96 h,EC50)为6.85 mg·L-1,水稻ρ(96 h,EC50)为11.27 mg·L-1,表明再力花秸秆浸提液对水绵的毒性强于水稻。水绵细胞中MDA累积含量与水稻浸提液浓度之间呈显著正相关(P<0.01,r=0.977),与再力花浸提液浓度之间亦呈显著正相关(P<0.01,r=0.964)。各浓度再力花浸提液处理组水绵细胞中MDA累积含量均高于水稻处理组。当ρ(浸提液)为20 mg·L-1时,再力花处理组MDA累积含量最高,达1.884μmol·g-1,比相同浓度水稻浸提液处理组MDA累积含量高105.20%;电解质外渗率也呈现基本相同的变化趋势,即除ρ(浸提液)为0和4 mg·L-1以外,其他4个再力花浸提液浓度组水绵电解质外渗率均高于相应浓度水稻浸提液组。水绵的PSⅡ最大光能转换效率(F v/F m)、PSⅡ实际光能转化效率(ΦPSⅡ)和最大电子传递效率(ηe,t,max)的变化与浸提液浓度之间均呈负相关关系。将ρ(浸提液)为20 mg·L-1时与0 mg·L-1时相比,水稻处理组水绵F v/F m下降96.58%,再力花处理组下降82.24%;水稻处理组水绵ΦPSⅡ下降96.74%,再力花处理组下降96.17%;水稻处理组水绵ηe,t,max下降84.56%,再力花处理组下降43.40%。上述结果表明这2种植物秸秆浸提液对控制水绵的生长具有一定的潜在价值。
Effects of extracts, varying in concentration （4, 8, 12, 16, 20 mg·L-1 ） from Oryza sativa and Thalia deal- bata straws inhibiting growth of Spirogyra sp. were studied. Results show that the extracts in all the treatments displayed in- hibitory effects on Spirogyra sp. after 96 h of exposure, with content of chlorophyll in Spirogyra sp. cells decreasing with the increasing of extract concentration, demonstrating a significant dose-effect relationship. The 96 h, EC50 of T. dealbata extract was 6. 85 mg ·L-1 and of O. sativa extract, 11.27 mg ·L-1, obviously showing that the former is higher than the latter in toxicity to Spirogyra sp.. Extremely significant positive relationships were observed between MDA content in Spiro- gyra sp. cells and concentration of O. sativa extract （P〈0. 01,r = 0. 977）, and between MDA content and concentration of T. dealbata extract （P〈0. 01, r = 0. 964）, too. MDA contents were higher in T. dealbata treatments than in O. sativa treat- ments, regardless of extract concentration. Among all the treatments, the one of T. dealbata extract, 20 mg ~ L-l in con- centration, had the highest MDA content, reaching 1. 884 p^mol ~ g-~ or being 105.20% higher than that in the treatment of O. sativa extract the same in concentration. A similar trend was also observed with electrolyte exosmosis （REL） , that is to say, REL is higher in all the T. dealbata treatments than in their corresponding O. sativa treatments, except for the treat- ments 0 or 4 mg· L-1 in extract concentration. The PSⅡ maximal photochemical efficiency （ Fv/Fm ）, the PS Ⅱ actual photochemical efficiency （ФPSⅡ） and the maximal relative electron transport rate （ηe,t,max） of Spirogyra sp. were found to be negatively related to concentration of the extracts. In the treatments of O. sativa extract, Fv/Fm of Spirogyra sp. was 96. 58%, ФPSⅡ, 96. 74% lower and ηe,t,max 84. 56% lower in the treatment, 20 mg· L-1 in concentration than in that 0 mg · L-1 in concentration, while in the treatments of T. dealbata extract FJF,~ was 82. 24% lower,ФPSⅡ, 96. 17% lower and ηe,t,max 43.40% lower in the former than in the latter. All the above findings suggest that the extracts of the straws of the two plants have some potential value in controlling growth of Spirogyra sp. in water environment.
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2014年第1期57-62,共6页
Journal of Ecology and Rural Environment
基金
国家水体污染控制与治理科技重大专项(2012ZX07101-007)
关键词
植物秸秆浸提液
水绵
抑制作用
叶绿素荧光参数
plant straw extract
Spirogyra sp.
inhibitory effect
chlorophyll fluorescence parameter
