摘要
为研究六价铬（Cr^6＋）对水绵（Spirogyrasp．）的毒性效应和水绵对六价铬的毒性响应，实验设置5个暴露组（4、6、8、10和12mg／L）和1个对照组。结果表明：96h后各暴露组对水绵的生长均有抑制作用，铬浓度越高，藻细胞叶绿素a越低，显示出较明显的剂量一效应关系；六价铬对水绵的96h的Ec。值为7．25mg／L。细胞浸出液电导率在低浓度（4～6mg／L）组上升较缓慢，在高浓度组（8～12mg／L）上升显著；丙二醛（MDA）累积含量在Cr6＋≥4mg／L时，累计含量上升较高（P〈0．01），当Cr^6＋≥8mg／L时，累积含量上升较少。水绵细胞浸出液电导率与MDA存在显著正相关（P〈0．05，r=0．891）。水绵细胞浸出液电导率和MDA含量与六价铬浓度分别存在显著（P〈0．05，r=0．951）和极显著（P〈0．01，r=0．977）的非线性的毒性响应关系。
To assess the toxicity of Cr6 ＋ on Spirogyra sp. and the response of Spirogyra sp. to Cr6 ＋ , five exposure groups and one con- trol group were investigated. The result showed that Cr6＋ had inhibitory effect on Spirogyra sp. after 96 h in all exposure groups. The chlorophyll-a of Spirogyra sp. decreased with the increase of extracts concentration in a clear dose-effect relationship. The 96 h EC50of Cr6＋ to Spirogyra sp. was 7.25 mg/L. Electrical conductivity extracts form of cell increased slowly on the low Cr6＋ concentration（4 - 6 rag/L） groups, but they increased significantly on the high Cr6 ~ concentration （ P 〈 0. 01 ）. The MDA concentration raise rapidly when Cr6＋ concentration ≥4 mg/L（ to control group, P 〈 0. 01 ）, but slowly when Cr6＋ concentration I〉 8 mg/L. It has a significant positive correlation between electrical conductivity extracts form of cell with MDA（ P 〈 0.05, r = 0. 891 ）. It has a significant positive correlation between electrical conductivity extracts form of cell with Cr6＋ concentration（P 〈 0.05 ,r = 0.951 ）, and has a great signifi- cant positive correlation between MDA with Cr6＋ concentration（P 〈 0.01 ,r = 0. 977）.
出处
《生物学杂志》
CAS
CSCD
2014年第1期24-27,共4页
Journal of Biology
基金
国家水体污染控制与治理科技重大专项(2012ZX07101-007)
