摘要
目的 研究肺水通道蛋白 1,4(AQP1,4)对肺泡毛细血管间水通透性和压力性肺水肿的影响 ,肺泡液体主动转运对肺泡液体清除 (AFC)的影响 ,以及不同pH条件下大鼠AFC的变化。方法 采用表面荧光技术和重力方法研究小鼠肺泡毛细血管间水通透性及水通道蛋白与压力性肺水肿的关系 ;测定大鼠肺泡灌注液中同位素标记白蛋白浓度推测AFC变化。应用低pH溶液灌注大鼠肺诱发肺损伤 ,并测定不同pH条件下AFC变化。结果 AQP1敲除可减轻小鼠肺泡毛细血管间水通透性和压力性肺水肿的程度。沙丁胺醇可促进AFC ,合用心得安可抑制其作用。当大鼠肺泡灌注液pH >2 5时 ,间羟叔丁肾上腺素、异丙肾上腺素、沙美特罗均可促进AFC ,Na通道抑制剂Benzamil可抑制AFC。结论 AQP1敲除明显降低小鼠肺泡毛细血管间水通透性 ,轻度减轻压力性肺水肿。β肾上腺能激动剂促进机械通气大鼠AFC。AFC的前提是完整肺泡上皮的存在 ,当pH低于 2 5时 ,促进AFC的作用被肺损伤作用掩盖。
Objective To explore the effects of aquaporins (AQP) 1,4 on water transport between airspace and pulmonary capillary and high pressure pulmonary edema The other purpose was to study the active alveolar fluid transport as well as alveolar fluid clearance (AFC) in different pH of airspace. Methods The effects of AQP1, 4 on water transport between airspace and pulmonary capillary and high pressure pulmonary edema were studied by surface fluorescence methods and gravimetric method respectively, AFC and AFC in airspace with different pH were measured by isotope method. Results AQP1 knockout inhibited water transport through pulmonary capillary and also reduced high pressure pulmonary edema Salbutamol increased AFC, but it was reduced when propranolol was used together AFC was improved by terbutaline, isoproterenol, salmeterol when pH >2 5 in airspace, but the effect was reduced after pH <2 5 in airspace Sodium channel inhibitor, Benzamil, could also inhibit AFC. Conclusions AQP1 knockout significantly reduced water transport through alveolar capillary, and also reduced high pulmonary edema slightly β adrenergic stimulant improved AFC in mechanically ventilated rats Intact airspace is very important for the function of AFC, however, the effect of AFC was impaired after lung injury induced by pH<2 5 in airspace
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2001年第2期105-108,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
上海市科技发展基金!资助项目 (0 0JC14 0 41)
