摘要
目的 探讨钙离子拮抗剂对离体肾的保护作用。方法 以 3月龄的日本纯种大耳兔为实验对象 ,分为正常组、实验对照组和实验组 ,实验对照组将肾脏置于 4℃高渗枸橼酸盐腺嘌呤器官保存液 (HC A液 )中保存 2 4h ,实验组将肾脏置于 4℃含维拉帕米的HC A液中保存 2 4h。正常组肾切取即刻测定肾细胞线粒体内钙离子及肾组织中三磷酸腺苷 (ATP)的含量 ;实验对照组和实验组保存 2 4h后测定肾细胞线粒体内钙离子及肾组织中ATP的含量。结果 与肾脏切取后立即检测组相比 ,实验对照组肾细胞线粒体内Ca2 +含量明显上升 (P <0 .0 1) ,ATP明显下降 (P <0 .0 1) ;实验组上述改变可得以显著改善 (P <0 .0 1)。结论 在离体肾保存中 ,应用钙离子拮抗剂维拉帕米可阻止Ca2 +进入细胞线粒体内 ,防止能量消耗 。
Objective To investigate the protective effect of calcium antagonists on kidneys stored in HC A solution in the model of rabbit.Methods The isolated kidneys from rabbits were perfused with and then stored in HC A solution at 4?℃ for 24?h. HC A solution containing verapamil (VP, a calcium antagonist) was added in some cases. The contents of mitochondrial calcium and renal tissue's ATP were measured in each group. Results The contents of mitochondrial calcium was remarkably higher and ATP was significantly lower in kidneys stored in HC A solution at 4?℃ for 24?h than in the normal control. If the HC A solution containing VP was added, the changes mentioned above could be significantly hindered. Conclusion Calcium antagonists is beneficial to isolated kidney during its preservation in HC A solution.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2001年第1期52-53,共2页
Chinese Journal of Organ Transplantation
关键词
器官保存
肾脏
钙通道
腺苷三磷酸
Organ preservation
Kidney
Calcium channels
Adenosine triphosphates
