摘要
利用离体大鼠肾灌流模型研究了ＦＫ５０６的肾毒性，ＦＫ５０６的灌流浓度分别为１０－８ｍｏｌ／Ｌ、１０－６ｍｏｌ／Ｌ、及１０－５ｍｏｌ／Ｌ。结果显示：１０－５ｍｏｌ／Ｌ的ＦＫ５０６灌流后肾灌流阻力、灌流量及肾小球滤过率均有明显改变，１５ｍｉｎ、３０ｍｉｎ及４５ｍｉｎ三指标分别上升或下降２２．２％～３２．５％，与基础值及对照组相比均有显著性差异（Ｐ＜０．０１～Ｐ＜０．００１）。结果同时显示短期灌流后对钠、水回吸收无影响。作者认为ＦＫ５０６对肾脏的急性作用主要影响肾血管。Ｐ＜０．００１；组间比较△Ｐ＜０．０１．△△Ｐ＜０．００１２．３不同浓度ＦＫ５０６对钠及水回吸收的影响 由表２可见，用１０－８ｍｏｌ／Ｌ、１０－６ｍｏｌ／Ｌ、及１０－５ｍｏｌ／Ｌ的ＦＫ５０６灌流后水的回吸收略有增加，但无统计学意义。表２不同浓度ＦＫ５０６对肾钠、水回吸收分数的影响组间组内比较均无显著差异２．４不同浓度ＦＫ５０６对肾灌流阻力的影响由表３可见，用１０－５ｍｏｌ／ＬＦＫ５０６灌流后，肾灌流阻力明显上升，１５ｍｉｎ、３０ｍｉｎ及４５ｍｉｎ分别上升２６．５％、２５％及２９％。与对照组及同组基础值相比差异有显著性（Ｐ均＜０．０１）。表３不同浓?
Objective: To study the direct toxic effect of FK506 on kidneys. Methods: The technique of IPRK (isolated perfused rat kidney) was used. The perfusate contained FK506 of varied concentrations, 10-8mol/L, 10-6mol/L and 10-5 mol/L. The perfusion pressure was adjusted to and stablized at 13. 3kPa for 30 min to take the baseline readings before starting the controlled experiment. The urine specimens were weighed, the flow rate of perfusate was read directly. Urine sodium and creatinine were determined using conventional methods. Results: Calculation of the data obtained at 15min, 30min and 45min revealed that only 10-5mol/L FK506 affected the kidney. As compared with their own baseline values and the values of other groups, the perfusion resistance was increased and the perfusate flow rate and glomerular filtration rate were decreased significantly by 22. 2%～32. 5% (P<0.01～P (0.001). The changes in tubular reabsorption of water and sodium were insignificant. Conclusion: The acute direct nephrotoxic effect of FK506 is apparently on the vascular bed, with the tubules’function uninvolved, at least in the acute phase.
出处
《徐州医学院学报》
CAS
1996年第4期362-365,共4页
Acta Academiae Medicinae Xuzhou
