摘要
为探讨姜对高温胁迫的生理响应特性,采用砂培法,研究了高温对姜叶片活性氧代谢的影响及培养液添加NO供体硝普钠(SNP)缓解高温胁迫的作用。结果表明,高温胁迫显著降低姜叶片色素含量,增加产生速率及H2O2和MDA含量;叶片SOD、POD、CAT等抗氧化酶活性在高温胁迫初期增强,随胁迫时间延长急剧下降;同时,AsA–GSH循环遭受破坏。外源添加0.1 mmol·L-1 SNP能显著增强姜叶片的抗氧化酶活性,降低叶片ROS水平,减轻膜脂过氧化程度,维持AsA–GSH循环系统的稳定性;降低叶片色素降解,有效缓解高温对姜叶片造成的伤害,增强其耐热性。
To investigate the physiological response characteristics of ginger under heat stress, a sandy-cultured experiment was conducted to study the effects of high temperature on active oxygen metabolism of ginger leaves and the mitigation effect of SNP （sodium nitroprusside, a NO donor） application in ginger leaves under heat stress. Such as pigments content, reactive oxygen species level, enzymatic and non-enzymatic antioxidant systems were considered in this study. A significant decrease of pigments content as well as accumulation of O2, H202 and MDA were observed in heat-stressed ginger leaves. Additionally, SOD, POD and CAT activities were enhanced at the early stage of heat stress, which were then sharply inhibited with the stress time prolonging and blocked AsA - GSH cycle. Compared with the control treatment, application of 0.1 mmol · L-1 SNP significantly enhanced activities of antioxidant enzymes as well as palingenesis of AsA - GSH cycle that significantly decreased levels of reactive oxygen species and membrane lipid peroxidation. Furthermore, application of SNP conferred ginger plants heat stress tolerance by pigment contents analysis.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第2期277-284,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31171953)
关键词
姜
高温胁迫
一氧化氮
活性氧代谢
ginger： Zingiberofficinale： heat stress： nitric oxide： reactive oxygenmetabolism
