摘要
目的观察miR-21抑制剂在5-氟尿嘧啶(5-FU)抑制肠癌细胞增殖中的作用,并探讨其分子机制。方法采用qRTPCR技术检测肠癌组织中miR-21的表达;应用CCK-8试剂盒检测肠癌细胞的增殖活力;运用Western blot法检测细胞中PTEN和AKT的磷酸化表达水平。结果肠癌组织中,miR-21的表达高于正常大肠组织;利用miR-21抑制剂联合5-FU处理肠癌细胞HT-29和LoVo,细胞增殖活力显著低于单独使用5-FU处理组(P<0.001);Western blot检测结果表明:miR-21抑制剂联合5-FU处理细胞后,抑癌基因PTEN的表达水平升高,AKT的磷酸化水平降低。结论 miR-21抑制剂可显著增强肠癌细胞对5-FU的敏感性。
Purpose To investigate the suppression of miR-21 inhibitor combined with 5-FU in intestinal carcinoma and the molecular mechanisms. Methods Expression of miR-21 in intestinal carcinoma was analyzed by real-time PCR. Cell proliferation was deter- mined using CCK-8 kit. Expression of PTEN and phosphorylation of AKT was evaluated by Western blot. Results Expression of miR- 21 in intestinal carcinoma was higher than that in normal intestine. In contrast to 5-FU treatment alone, miR-21 inhibitor combined with 5-FU could suppress intestinal carcinoma cell lines HT-29 and LoVo more significantly in vitro （ P 〈 0. 001 ）. Tumor suppressor gene FFEN was up-regulated while phosphorylation of AKT was down-regulated after combining treatment of miR-21 inhibitor and 5- FU, as showed with Western blot. Conclusion MiR-21 inhibitor can enhance the sensitivity of intestinal carcinoma cells to 5-FU.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2014年第2期131-134,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
江苏大学临床医学科技发展基金(JLY2010150)
