摘要
目的建立腹泻型肠易激综合征(IBS-D)大鼠模型,从大鼠一般情况、大便性状、腹壁撤退反射(abdominal withdrawal reflex,AWR)评分及组织病理学观察温肾健脾法干预IBS-D的效果。方法 72只大鼠随机分为正常组、模型组、温肾健脾方高、中、低剂量组和对照组共6组,每组12只。参照AL-Chaer ED造模方法成功建立IBS-D大鼠模型,造模结束后,治疗组分别予温肾健脾方免煎剂高、中、低剂量灌胃治疗,对照组予四神丸方免煎剂灌胃,模型组予等体积生理盐水灌胃,给药2周。观察用药前后大鼠一般情况、大便性状、大鼠AWR及组织病理学改变。结果治疗后各组大鼠一般情况均有不同程度的好转,温肾健脾方高、中剂量组大鼠的改善情况优于温肾健脾方低剂量组和对照组(P<0.05)。治疗前后各组大鼠体重相对增长率比较,差异无统计学意义(P>0.05)。治疗后与模型组及温肾健脾方低剂量组比较,温肾健脾方高、中剂量组和对照组大鼠在4 h内排便粒数减少,差异均有统计学意义(P<0.05)。治疗后温肾健脾方高、中剂量组大鼠4 h内粪便Bristol分级评分、平均稀便级和粪便干湿比重均低于对照组和温肾健脾方低剂量组(P<0.05)。在球囊扩张的容量为1.5 mL时,温肾健脾方高、中剂量组AWR评分低于对照组和温肾健脾方低剂量组(P<0.05);各组大鼠组织标本大体观与病理学观察均未见器质性改变。结论此批模型大鼠进行内脏高敏感性IBS-D研究可靠;温肾健脾法能减少IBS-D大鼠排便粒数、降低Bristol分级评分、平均稀便级和粪便干湿比重,在一定程度能降低大鼠内脏高敏感性。
Objective IBS-D rat model was established to assess the effect of Shen warming Pi strengthening method （SWPSM） for intervening diarrhea-predominant irritable bowel syndrome （IBS-D） by observing rats＇ general state, stool properties, AWR ranking, and histopathological changes. Methods Totally 72 rats were randomly divided into 6 groups, i.e. the normal group, the model group, the high, middle, low dose SWPSM groups, and the control group, 12 in each group. The IBS-D rat model was suc- cessfully established referring to AL-Chaer ED＇s modeling method. After modeling high, middle, and low dose SWPS Recipe boil-free granules were given by gastrogavage to rats in corresponding treatment groups. Sishen Pill boil-free granule was given by gastrogavage to those in the control group. Equal vol- ume of normal saline was given by gastrogavage to rats in the model group. The medication lasted for 2 weeks. Rats＇ general state, stool properties, abdominal withdrawal reflex （AWR） ranking, and his- topathological changes were observed. Results After treatment, the general state of all rats got im-provement to various degrees. The improvement in the high and middle dose SWPS Recipe groups were superior to that in the low dose SWPS Recipe group and the control group （P 〈0.05）. There was no sta- tistical difference in the growth rate between after and before treatment in each group （P ~0.05）. Com- pared with the model group and the low dose SWPS Recipe group, the defecation amount within 4 h was less in the high and middle dose SWPS Recipe groups and the control group （P 〈0.05）. The Bristol rank- ing score, average ranking of loose stool, ratio of dry stool and wet stool were lower in the high and mid- dle dose SWPS Recipe groups than in the control group and the low dose SWPS Recipe group （P 〈0.05）. The AWR ranking score was lower in the high and middle dose SWPS Recipe groups than in the control group when the volume of balloon dilation was 1.5 mL. There was no organic change of histological or morphological observation. Conclusions High sensitive IBS-D model was proved to be reliable. SWPSM could reduce the quantity of stools, lower Bristol ranking score, average ranking of loose stools as well as ratios of dry stool and wet stool, contributing to reducing the high sensitivity of rats＇ visceral organs to some extent.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2014年第2期197-202,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助课题(No.81072800)
关键词
肠易激综合征
温肾健脾法
irritable bowel syndrome
Shen warming Pi strengthening method
