MCP-1、ICAM-1在糖尿病肾病大鼠肾脏损害中作用及厄贝沙坦干预的影响被引量：22

The Role of MCP-1 and ICAM-1 in Kidney Damaged Diabetic Nephropathy Rats and the Intervention Effect of Irbesartan

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摘要目的:探讨MCP-1、ICAM-1在糖尿病肾病大鼠肾脏损害中作用及厄贝沙坦对二者的影响。方法:采用高糖高脂饮食合并链脲佐菌素腹腔注射的方法建立糖尿病肾病大鼠模型。将大鼠随机分为正常对照NC组、糖尿病肾病DN组、厄贝沙坦DI组,检测各组大鼠24 h尿量、24 h尿白蛋白定量(24 h UTP)、血糖(BG)、血肌酐(Scr)、尿素氮(BUN)、肾重指数(KI);行HE染色观察各组大鼠病理学形态,免疫组化观察MCP-1、ICAM-1蛋白的表达,RT-PCR观察MCP-1 mRNA、ICAM-1mRNA的表达。结果:与NC组比较,DN组大鼠肾脏病理改变加重,24 h尿量、24 h UTP、KI、BG、Scr、BUN、肾脏组织中MCP-1mRNA和ICAM-1mRNA及蛋白水平均显著增加,差异均有统计学意义(P<0.01);与DN组比较,DI组大鼠BG、BUN、Scr有所改善,差异无统计学意义;肾脏病理改变减轻,其余指标明显降低,差异有统计学意义(P<0.05)。结论:MCP-1、ICAM-1在糖尿病肾病肾脏损害过程中可能起重要作用;厄贝沙坦能够减轻糖尿病肾病肾组织MCP-1、ICAM-1的表达,缓解了肾脏病理损伤。 Objective： This experiment studied the role of monocyte chemoattractant protein - 1 （ MCP - 1 ） and intercellular adhesion molecule - 1 （ ICAM - 1 ） in Kidney damaged diabetic nephropathy rats and the effect of irbesartan on them. Methods： Using the high - sugar and high - fat diets combined with intraperitoneal injection of Streptozotocin to establish diabetic nephropathy rats model. Rats were randomly divided into normal control NC group, diabetic nephropathy DN group, irbesartan DI group ,Testing 24 h urine volume, 24 h urine protein quantitative （24 h UTP） , blood glucose （BG）, blood muscle anhydride （Ser）, urea nitrogen （ BUN）, and index of kidney （ renal/weight, KI） ; Using HE stain to observe pathological morphology of each group, immunohisto- chemistry to observe the protein expression of MCP - 1 and ICAM - 1, RT - PCR to observe their mRNA expression. Results： Com- pared with the NC group, kidney pathological changes of DN group aggravated, 24 h urine volume, 24 h UTP, BG, Scr, BUN, KI and expressions of MCP - 1 and ICAM - 1 mRNA and protein in the renal tissues were significantly increased , with statistically significant differences（ P 〈 0.01 ） ; Compared with the DN group, BG, Scr and BUN of DI group relatively improved , with no statistically sig- nificant differences. Kidney pathological changes alleviated. The rest indices evidently reduced, with statistically significant differ- ences （ P 〈 0.05 ）. Conclusion： MCP - land ICAM - 1 may play an important role in the process of kidney damage in diabetic nephropathy ; Irbesartan can reduce expressions of MCP - 1 and ICAM - 1 in diabetic renal tissues, to some extent relieving the pathological damage of the kidney.

作者张曼丽陈卫东杨萍常保超郭亚玲刘磊

机构地区蚌埠医学院第一附属医院肾内科

出处《中国中西医结合肾病杂志》 2013年第12期1040-1043,I0009,共5页 Chinese Journal of Integrated Traditional and Western Nephrology

基金安徽省高等学校省级自然科学研究项目(No.KJ2013A191)

关键词单核细胞趋化因子-1 细胞间黏附分子-1 糖尿病肾病肾脏损害厄贝沙坦 Monocyte chemoattractant protein - 1 Intercellular adhesion molecule - 1 Diabetic nephropathy Kidney damage Irbesartan

分类号 R587.2 [医药卫生—内分泌]

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参考文献15

1Navarro - Gonz61ez JF, Mora - Fem(mdez C. The role of inflam- matory cytokines in diabetic nephropathy. J Am Soc Nephrol, 2008,19(2) :433 -442.
2Jiao B, Wang YS, Cheng YN, et al. Valsartan attenuated oxida- tivestress decreased MCP - 1 and TGF - 131 expression in glo- merular mesangial and epithelial cells induced by high - glucoselevels. Biosci Trends,2011,5 (4) : 173.
3李伟,吴智勇,李娥卿.2型糖尿病患者血清可溶性细胞间粘附分子-1和可溶性E-选择素水平变化的观察[J].中华老年医学杂志,2003,22(6):370-370. 被引量：6
4杨亦彬,张翥,苏克亮,陈泽君,黄颂敏.链脲佐菌素诱导大鼠糖尿病肾病模型的方法学探讨[J].华西医学,2005,20(2):299-300. 被引量：51
5刘艳春,刘佳.糖尿病肾病的发病机制及治疗[J].国际内分泌代谢杂志,2011,31(2):84-86. 被引量：11
6胡承,虞伟慧,贾伟平.中国人2型糖尿病遗传学研究的现状——兼谈中西方人群差异[J].中华糖尿病杂志,2012,4(1):4-6. 被引量：12
7李妍妍,陆菊明,王淑玉,卢艳慧,宋瑛,刘力生,田慧,潘长玉.微量白蛋白尿对心血管疾病死亡及全因死亡风险的预测价值[J].中华糖尿病杂志,2011,3(4):319-323. 被引量：8
8Oliver L, Alessia F, Adeel L, et al. Role of inflammation in dia- betic nephropathy. Curr Diatbetes Rev ,2008,4 ( 1 ) :7.
9Frank CB, Charbel CK, Carolyn LB, et al. Abnormalities in signa- ling pathways in diabetic nephropathy. Expert Rev Endocrinol Metab,2010,5 ( 1 ) :51.
10覃丹平,刘岩.醛固酮在糖尿病肾病中的作用及其阻滞剂的益处[J].实用全科医学,2008,6(5):518-519. 被引量：8

二级参考文献72

1于德民,吴锐,尹潍,袁咏.实验性链脲佐菌素糖尿病动物模型的研究[J].中国糖尿病杂志,1995,3(2):105-109. 被引量：424
2Zoja C, Garcia PB, Remuzzi G. The role of chemokines in progressive renal disease. Front Biosci, 2009,14:1815 - 1822.
3Wang LJ, Zhang L, Yu Y, et al. The protective effects of taurine against early renal injury in STZ - induced diabetic rats, correlated with inhibition of renal LOX - 1 - Mediated ICAM - 1 expression. Ren Fail, 2008,30(8) : 763 - 771.
4Rodriguez - Iturbe B, Ferrebuz A, Vanegas V, et al. Early and Sustained Inhibition of NF- κB Prevents Hypertension in Spontaneously Hypertensive Rats. Pharmacol Exp Ther, 2005, 315 (1):51-57.
5Thomas M, Linda D. Effects of TNFα on Expression of ICAM - 1 in Human Airway Epithelial Cells in Vitro: Oxidant - Mediated Pathways and Transcription. Free Radical Biology & Medicine, 2003,35 (9) : 1158 - 1167.
6Wen Y, Skidmore JC, Porter - Turner MM, et al. Relationship of glycation, antioxidant status and oxidative stress to vascular endothelial damage in diabetes. Diabetes Obes Metab, 2002, 4 (5) : 305 - 308.
7Caroline SP,Dider H,Chantal M, et al. Early glomerular macrophage recruitment in streptozotocin- induced diabetic rats. Diabetes, 2000,49(3) :466- 475.
8Jude ED, Douglas JT, Anderson AG, et al. Circulating cellular adhesion molecules ICAM - 1, VCAM - 1, P - and E - selectin in the prediction of cardiovascular disease in diabetes mellitus. Euro J Intern Med,2002,13(3) : 185 - 189.
9Clayton A, Steadman R, Williams JD. Cells isolated from the human corticalinterstitium resemble myofibroblasts and bind neutrophils in an ICAM- 1 -dependent manner. Am Soc Nephrol, 1997,8(4) :604 - 615.
10Hikaru S,Kenichi S, Kyoji H, et al. Increased expression of intercellular adhesion molecule- 1 (ICAM- 1 )in diabetic rat glomerular hyperfiltration is a potential mechanism of ICAM - 1 upregulation. Diabetes, 1997,46 (12) : 2075 - 2081.